Treatment of murine CD5- B cells with anti-lg, but not LPS, induces surface CD5

Two B-cell activation pathways

Yingzi Cong, Evelyn Rabin, Henry H. Wortis

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Antl-lg stimulated murine B cells express high levels of surface CD5 (ly-1) and increased CD44 while maintaining surface IgD, CD23 and J11d. Sorting of CD5- and CD5+ cells demonstrates that anti-lg induces CD5 expression rather than the selective expansion of CD5+ cells. Anti Ig plus interieukln-6 (IL-6) induces the CD23, IgD, low ly-5 (B220) (CD45low), J11dhigh phenotype of typical CD5+ peritoneal B cells. In contrast, lipopolysaccharide (LPS)-stimulated B cells have high levels of CD44 but decreased surface IgD, CD23 and J11d and no CD5. Thus LPS and anti-lg generate activated cells with differing phenotypes. Induced CD5+ cells have increased viability, even in the absence of added exogenous factors, while the viability of CD5- B cells is dependent on factors such as IL-4. We conclude that conventional CD5- B cellscan be activated by either of two pathways: one generating CD5+ B cells; the other yielding conventional activated cells. We hypothesize that the first path requires slg cross-linking and corresponds to T-independent (type 2) stimulation, while cognate Interaction with helper T cells in the absence of slg cross-linking induces B cells to enter the second path.

Original languageEnglish (US)
Pages (from-to)467-476
Number of pages10
JournalInternational Immunology
Volume3
Issue number5
DOIs
StatePublished - May 1991
Externally publishedYes

Fingerprint

B Cells
Lipopolysaccharides
Activation
Pathway
B-Lymphocytes
Chemical activation
Cells
Immunoglobulin D
Cell
Viability
Phenotype
Linking
Path
T-cells
Helper-Inducer T-Lymphocytes
Sorting
Interleukin-4
Lipopolysaccharide
Express
Factors

Keywords

  • CD23
  • CD44
  • CD45
  • IL-4
  • IL-6
  • J11d
  • LgD
  • Ly-1
  • Ly-5
  • Lymphocytes
  • Pgp-1
  • Thymu-dependent antigent
  • Thymus-dependent antigent

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

Treatment of murine CD5- B cells with anti-lg, but not LPS, induces surface CD5 : Two B-cell activation pathways. / Cong, Yingzi; Rabin, Evelyn; Wortis, Henry H.

In: International Immunology, Vol. 3, No. 5, 05.1991, p. 467-476.

Research output: Contribution to journalArticle

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