Trichloroethylene induced deactivation of cytochrome P 450 and loss of liver glutathione in vivo

H. T. Moslen, E. S. Reynolds, P. J. Boor, K. Bailey, S. Szabo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Liver microsomal enzyme activities and glutathione (GSH) contents of fasted male rats pretreated with phenobarbital (PBT) or vehicle controls were measured during and after exposure to trichloroethylene (TRI) (1% x 2 hr). TRI caused morphologic liver injury only in the PBT animals. Cytochrome P 450 and b5 contents were diminished by the end of the first hr of TRI exposure and NADH cytochrome c reduction increased three fold by eight hr in the PBT animals. The only change in vehicle animals exposed to TRI was a decrease in NADPH cytochrome c reductase activity by eight hr. Hepatic GSH contents of vehicle animals, constant during TRI exposure, rose with time. In contrast, in PBT animals, hepatic GSH contents decreased during TRI exposure and then rebounded. Decreases in GSH were most profound in the microsomal fraction. When fed animals with ∞ two fold higher hepatic GSH levels than fasted animals were exposed to TRI, they had shorter anesthesia recovery times and less liver injury, although excreting similar or slightly more trichlorinated metabolite into their urine in 24 hr than their fasted counterparts. It is suggested that the hepatotoxic effects of trichloroethylene are caused by inadequate detoxification of its reactive intermediates.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume16
Issue number1
StatePublished - 1977

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Trichloroethylene
Liver
Cytochrome P-450 Enzyme System
Glutathione
Animals
Phenobarbital
NADPH-Ferrihemoprotein Reductase
Detoxification
Wounds and Injuries
Enzyme activity
Metabolites
Cytochromes c
NAD
Rats
Anesthesia
Urine
Recovery
Enzymes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Trichloroethylene induced deactivation of cytochrome P 450 and loss of liver glutathione in vivo. / Moslen, H. T.; Reynolds, E. S.; Boor, P. J.; Bailey, K.; Szabo, S.

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 16, No. 1, 1977, p. 109-120.

Research output: Contribution to journalArticle

Moslen, H. T. ; Reynolds, E. S. ; Boor, P. J. ; Bailey, K. ; Szabo, S. / Trichloroethylene induced deactivation of cytochrome P 450 and loss of liver glutathione in vivo. In: Research Communications in Chemical Pathology and Pharmacology. 1977 ; Vol. 16, No. 1. pp. 109-120.
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