Troglitazone inhibits progesterone production in porcine granulosa cells

Slavisa Gasic, Yvonne Bodenburg, Manubi Nagamani, Allan Green, Randall Urban

Research output: Contribution to journalArticle

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Abstract

Troglitazone (a thiazolidinedione that improves insulin resistance) lowers elevated androgen concentrations in women with polycystic ovarian syndrome. In this study, we assessed the direct effects of troglitazone on steroidogener, is in porcine granulosa cells. Troglitazone inhibited progesterone production in a dose- and time-dependent manner (earliest effects at 4 h, maximum at 24 h) without affecting cell viability. Progesterone production was also inhibited by troglitazone in the presence of 25-hydroxycholesterol, indicating that the drug does not affect intracellular cholesterol transport. Troglitazone also inhibited FSH- and forskolin- stimulated progesterone secretion. The reduced progesterone production was accompanied by marked elevations of pregnenolone concentrations, suggesting inhibition of 3β-hydroxysteroid dehydrogenase (3β-HSD). The activity of 3β-HSD in troglitazone-treated granulosa cells was decreased by more than 60%, compared with controls after 24 h. Troglitazone did not affect aromatase activity in porcine granulosa cells. In summary, troglitazone has direct effects on porcine granulosa cell steroidogenesis. The drug specifically inhibits 3β-HSD activity, resulting in impaired progesterone production. The clinical relevance of this direct in vitro effect on steroidogenesis needs further investigation.

Original languageEnglish (US)
Pages (from-to)4962-4966
Number of pages5
JournalEndocrinology
Volume139
Issue number12
StatePublished - 1998

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troglitazone
Granulosa Cells
Progesterone
Swine
3-Hydroxysteroid Dehydrogenases
Pregnenolone
Aromatase
Polycystic Ovary Syndrome
Colforsin
Pharmaceutical Preparations
Androgens
Insulin Resistance

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gasic, S., Bodenburg, Y., Nagamani, M., Green, A., & Urban, R. (1998). Troglitazone inhibits progesterone production in porcine granulosa cells. Endocrinology, 139(12), 4962-4966.

Troglitazone inhibits progesterone production in porcine granulosa cells. / Gasic, Slavisa; Bodenburg, Yvonne; Nagamani, Manubi; Green, Allan; Urban, Randall.

In: Endocrinology, Vol. 139, No. 12, 1998, p. 4962-4966.

Research output: Contribution to journalArticle

Gasic, S, Bodenburg, Y, Nagamani, M, Green, A & Urban, R 1998, 'Troglitazone inhibits progesterone production in porcine granulosa cells', Endocrinology, vol. 139, no. 12, pp. 4962-4966.
Gasic S, Bodenburg Y, Nagamani M, Green A, Urban R. Troglitazone inhibits progesterone production in porcine granulosa cells. Endocrinology. 1998;139(12):4962-4966.
Gasic, Slavisa ; Bodenburg, Yvonne ; Nagamani, Manubi ; Green, Allan ; Urban, Randall. / Troglitazone inhibits progesterone production in porcine granulosa cells. In: Endocrinology. 1998 ; Vol. 139, No. 12. pp. 4962-4966.
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