Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens

N. H. Ansari, A. Bhatnagar, E. Fulep, P. Khanna, S. K. Srivastava

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Abstract

Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman- 2-carboxylic acid (Trolox) provided good protection against 50 mM glucose- induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose + antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume84
Issue number1
StatePublished - Jan 1 1994

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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