Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman- 2-carboxylic acid (Trolox) provided good protection against 50 mM glucose- induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose + antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.
|Original language||English (US)|
|Number of pages||12|
|Journal||Research Communications in Chemical Pathology and Pharmacology|
|State||Published - Jan 1 1994|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)