Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens

Naseem Ansari, A. Bhatnagar, E. Fulep, P. Khanna, Satish Srivastava

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hyperglycemia-induced cataractogenesis has been studied in rat lenses cultured in 50 mM glucose using an inverted microscope connected with a Universal C-mount and a CCD camera. Digital images were acquired and the opacity was determined by quantitating the transmitted light. Antioxidants, butylated hydroxy toluene (BHT) and 6-hydroxy-2,5,7,8-tetramethenyl-chroman- 2-carboxylic acid (Trolox) provided good protection against 50 mM glucose- induced cataractogenesis in rat lenses for upto 8 days. Sorbitol levels in the 50 mM glucose + antioxidant groups were approximately 1.5 mM fold higher than in 50 mM glucose. The results, besides further demonstrating that oxidative damage is the major mechanism of sugar-induced cataractogenesis, show that Trolox or related amphipathic compounds could be of therapeutic use in the prevention of diabetic cataracts.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume84
Issue number1
StatePublished - 1994

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Hyperglycemia
Lenses
Rats
Glucose
Chromans
Antioxidants
Sorbitol
Opacity
Toluene
Therapeutic Uses
CCD cameras
Carboxylic Acids
Sugars
Cataract
Microscopes
Light
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens. / Ansari, Naseem; Bhatnagar, A.; Fulep, E.; Khanna, P.; Srivastava, Satish.

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 84, No. 1, 1994, p. 93-104.

Research output: Contribution to journalArticle

Ansari, Naseem ; Bhatnagar, A. ; Fulep, E. ; Khanna, P. ; Srivastava, Satish. / Trolox protects hyperglycemia-induced cataractogenesis in cultured rat lens. In: Research Communications in Chemical Pathology and Pharmacology. 1994 ; Vol. 84, No. 1. pp. 93-104.
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