Tropisetron increases the inhibitory effect of mild restraint on lordosis behavior of hormonally primed, ovariectomized rats

Ovariectomized rats, hormonally primed with 10μg estradiol benzoate and 500μg progesterone are resistant to the lordosis-inhibiting effects of a 5min restraint experience. However, modulation of the serotonergic (5-HT) system alters this resistance to stress. In the following experiment, ovariectomized Fischer inbred rats were hormonally primed with 10μg estradiol benzoate and 500μg progesterone. The effect of 5min restraint on sexual behavior was examined after bilateral hypothalamic infusion or intraperitoneal (ip) treatment with the 5-HT ₃ receptor antagonist, 3-tropanylindole-3-carboxylate hydrochloride (tropisetron). Infusion with 50 or 100ng tropisetron inhibited lordosis behavior. When rats were infused with 10 or 25ng tropisetron, rats showed normal lordosis behavior. However, when infusion with 10 or 25ng tropisetron was combined with 5min restraint, lordosis behavior was inhibited. These findings are consistent with prior work that has implicated hypothalamic serotonin in control of lordosis behavior and in the effect of mild restraint on the behavior. In contrast to the effects of the intracranial infusion, intraperitoneal injection with 1.0 or 2.0mg/kg tropisetron did not amplify the effects of restraint.