TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi

Utpal Pal, Xin Li, Tian Wang, Ruth R. Montgomery, Nandhini Ramamoorthi, Aravinda M. DeSilva, Fukai Bao, Xiaofeng Yang, Marc Pypaert, Deepti Pradhan, Fred S. Kantor, Sam Telford, John F. Anderson, Erol Fikrig

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

The Lyme disease agent Borrelia burgdorferi naturally persists in a cycle that primarily involves ticks and mammals. We have now identified a tick receptor (TROSPA) that is required for spirochetal colonization of Ixodes scapularis. B. burgdorferi outer surface protein A, which is abundantly expressed on spirochetes within the arthropod and essential for pathogen adherence to the vector, specifically bound to TROSPA. TROSPA mRNA levels in ticks increased following spirochete infestation and decreased in response to engorgement, events that are temporally linked to B. burgdorferi entry into and egress from the vector. The blockade of TROSPA by TROSPA antisera or by the repression of TROSPA expression via RNA interference reduced B. burgdorferi adherence to the I. scapularis gut in vivo, thereby preventing efficient colonization of the vector and subsequently reducing pathogen transmission to the mammalian host. Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature.

Original languageEnglish (US)
Pages (from-to)457-468
Number of pages12
JournalCell
Volume119
Issue number4
DOIs
StatePublished - Nov 12 2004
Externally publishedYes

Fingerprint

Ixodes
Borrelia burgdorferi
Spirochaetales
Ticks
Pathogens
Arthropods
Mammals
Infectious Disease Transmission
Lyme Disease
Immune Sera
RNA Interference
RNA
Ligands
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Pal, U., Li, X., Wang, T., Montgomery, R. R., Ramamoorthi, N., DeSilva, A. M., ... Fikrig, E. (2004). TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell, 119(4), 457-468. https://doi.org/10.1016/j.cell.2004.10.027

TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. / Pal, Utpal; Li, Xin; Wang, Tian; Montgomery, Ruth R.; Ramamoorthi, Nandhini; DeSilva, Aravinda M.; Bao, Fukai; Yang, Xiaofeng; Pypaert, Marc; Pradhan, Deepti; Kantor, Fred S.; Telford, Sam; Anderson, John F.; Fikrig, Erol.

In: Cell, Vol. 119, No. 4, 12.11.2004, p. 457-468.

Research output: Contribution to journalArticle

Pal, U, Li, X, Wang, T, Montgomery, RR, Ramamoorthi, N, DeSilva, AM, Bao, F, Yang, X, Pypaert, M, Pradhan, D, Kantor, FS, Telford, S, Anderson, JF & Fikrig, E 2004, 'TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi', Cell, vol. 119, no. 4, pp. 457-468. https://doi.org/10.1016/j.cell.2004.10.027
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, DeSilva AM et al. TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell. 2004 Nov 12;119(4):457-468. https://doi.org/10.1016/j.cell.2004.10.027
Pal, Utpal ; Li, Xin ; Wang, Tian ; Montgomery, Ruth R. ; Ramamoorthi, Nandhini ; DeSilva, Aravinda M. ; Bao, Fukai ; Yang, Xiaofeng ; Pypaert, Marc ; Pradhan, Deepti ; Kantor, Fred S. ; Telford, Sam ; Anderson, John F. ; Fikrig, Erol. / TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. In: Cell. 2004 ; Vol. 119, No. 4. pp. 457-468.
@article{db8ffae2564341898ff6e350714a1fdc,
title = "TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi",
abstract = "The Lyme disease agent Borrelia burgdorferi naturally persists in a cycle that primarily involves ticks and mammals. We have now identified a tick receptor (TROSPA) that is required for spirochetal colonization of Ixodes scapularis. B. burgdorferi outer surface protein A, which is abundantly expressed on spirochetes within the arthropod and essential for pathogen adherence to the vector, specifically bound to TROSPA. TROSPA mRNA levels in ticks increased following spirochete infestation and decreased in response to engorgement, events that are temporally linked to B. burgdorferi entry into and egress from the vector. The blockade of TROSPA by TROSPA antisera or by the repression of TROSPA expression via RNA interference reduced B. burgdorferi adherence to the I. scapularis gut in vivo, thereby preventing efficient colonization of the vector and subsequently reducing pathogen transmission to the mammalian host. Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature.",
author = "Utpal Pal and Xin Li and Tian Wang and Montgomery, {Ruth R.} and Nandhini Ramamoorthi and DeSilva, {Aravinda M.} and Fukai Bao and Xiaofeng Yang and Marc Pypaert and Deepti Pradhan and Kantor, {Fred S.} and Sam Telford and Anderson, {John F.} and Erol Fikrig",
year = "2004",
month = "11",
day = "12",
doi = "10.1016/j.cell.2004.10.027",
language = "English (US)",
volume = "119",
pages = "457--468",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi

AU - Pal, Utpal

AU - Li, Xin

AU - Wang, Tian

AU - Montgomery, Ruth R.

AU - Ramamoorthi, Nandhini

AU - DeSilva, Aravinda M.

AU - Bao, Fukai

AU - Yang, Xiaofeng

AU - Pypaert, Marc

AU - Pradhan, Deepti

AU - Kantor, Fred S.

AU - Telford, Sam

AU - Anderson, John F.

AU - Fikrig, Erol

PY - 2004/11/12

Y1 - 2004/11/12

N2 - The Lyme disease agent Borrelia burgdorferi naturally persists in a cycle that primarily involves ticks and mammals. We have now identified a tick receptor (TROSPA) that is required for spirochetal colonization of Ixodes scapularis. B. burgdorferi outer surface protein A, which is abundantly expressed on spirochetes within the arthropod and essential for pathogen adherence to the vector, specifically bound to TROSPA. TROSPA mRNA levels in ticks increased following spirochete infestation and decreased in response to engorgement, events that are temporally linked to B. burgdorferi entry into and egress from the vector. The blockade of TROSPA by TROSPA antisera or by the repression of TROSPA expression via RNA interference reduced B. burgdorferi adherence to the I. scapularis gut in vivo, thereby preventing efficient colonization of the vector and subsequently reducing pathogen transmission to the mammalian host. Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature.

AB - The Lyme disease agent Borrelia burgdorferi naturally persists in a cycle that primarily involves ticks and mammals. We have now identified a tick receptor (TROSPA) that is required for spirochetal colonization of Ixodes scapularis. B. burgdorferi outer surface protein A, which is abundantly expressed on spirochetes within the arthropod and essential for pathogen adherence to the vector, specifically bound to TROSPA. TROSPA mRNA levels in ticks increased following spirochete infestation and decreased in response to engorgement, events that are temporally linked to B. burgdorferi entry into and egress from the vector. The blockade of TROSPA by TROSPA antisera or by the repression of TROSPA expression via RNA interference reduced B. burgdorferi adherence to the I. scapularis gut in vivo, thereby preventing efficient colonization of the vector and subsequently reducing pathogen transmission to the mammalian host. Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature.

UR - http://www.scopus.com/inward/record.url?scp=8344262276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8344262276&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2004.10.027

DO - 10.1016/j.cell.2004.10.027

M3 - Article

C2 - 15537536

AN - SCOPUS:8344262276

VL - 119

SP - 457

EP - 468

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -