TRPM4b channel suppresses store-operated Ca2+ entry by a novel protein-protein interaction with the TRPC3 channel

Jae Yong Park, Eun Mi Hwang, Oleg Yarishkin, Jin Ho Seo, Eunju Kim, Jiyun Yoo, Gwan Su Yi, Dong Gyu Kim, Nammi Park, Chang Man Ha, Jun-Ho La, Dawon Kang, Jaehee Han, Uhtaek Oh, Seong Geun Hong

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We identified human TRPC3 protein by yeast two-hybrid screening of a human brain cDNA library with human TRPM4b as a bait. Immunoprecipitation and confocal microscopic analyses confirmed the protein-protein interaction between TRPM4b and TRPC3, and these two TRPs were found to be highly colocalized at the plasma membrane of HEK293T cells. Overexpression of TRPM4b suppressed TRPC3-mediated whole cell currents by more than 90% compared to those in TRPC3-expressed HEK293T cells. Furthermore, HEK293T cells stably overexpressing red fluorescent protein (RFP)-TRPM4b exhibited an almost complete abolition of UTP-induced store-operated Ca2+ entry, which is known to take place via endogenous TRPC channels in HEK293T cells. This study is believed to provide the first clear evidence that TRPM4b interacts physically with TRPC3, a member of a different TRP subfamily, and regulates negatively the channel activity, in turn suppressing store-operated Ca2+ entry through the TRPC3 channel.

Original languageEnglish (US)
Pages (from-to)677-683
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number3
DOIs
StatePublished - Apr 11 2008
Externally publishedYes

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Uridine Triphosphate
Cell membranes
Gene Library
Yeast
Brain
Screening
Proteins
Immunoprecipitation
Yeasts
Cell Membrane
TRPC3 cation channel
red fluorescent protein

Keywords

  • Store-operated Ca entry
  • TRPC3
  • TRPM4b
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

TRPM4b channel suppresses store-operated Ca2+ entry by a novel protein-protein interaction with the TRPC3 channel. / Park, Jae Yong; Hwang, Eun Mi; Yarishkin, Oleg; Seo, Jin Ho; Kim, Eunju; Yoo, Jiyun; Yi, Gwan Su; Kim, Dong Gyu; Park, Nammi; Ha, Chang Man; La, Jun-Ho; Kang, Dawon; Han, Jaehee; Oh, Uhtaek; Hong, Seong Geun.

In: Biochemical and Biophysical Research Communications, Vol. 368, No. 3, 11.04.2008, p. 677-683.

Research output: Contribution to journalArticle

Park, JY, Hwang, EM, Yarishkin, O, Seo, JH, Kim, E, Yoo, J, Yi, GS, Kim, DG, Park, N, Ha, CM, La, J-H, Kang, D, Han, J, Oh, U & Hong, SG 2008, 'TRPM4b channel suppresses store-operated Ca2+ entry by a novel protein-protein interaction with the TRPC3 channel', Biochemical and Biophysical Research Communications, vol. 368, no. 3, pp. 677-683. https://doi.org/10.1016/j.bbrc.2008.01.153
Park, Jae Yong ; Hwang, Eun Mi ; Yarishkin, Oleg ; Seo, Jin Ho ; Kim, Eunju ; Yoo, Jiyun ; Yi, Gwan Su ; Kim, Dong Gyu ; Park, Nammi ; Ha, Chang Man ; La, Jun-Ho ; Kang, Dawon ; Han, Jaehee ; Oh, Uhtaek ; Hong, Seong Geun. / TRPM4b channel suppresses store-operated Ca2+ entry by a novel protein-protein interaction with the TRPC3 channel. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 368, No. 3. pp. 677-683.
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