Trypanosoma cruzi infection suppresses nuclear factors that bind to specific sites on the interleukin-2 enhancer

Lynn Soong, Rick L. Tarleton

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interleukin-2 (IL-2) gene expression, a critical early event during T lymphocyte activation, is severely suppressed in mice infected with the protozoan parasite Trypanosoma cruzi, the causative agent of human Chagas' disease. Our previous observation that reduction of IL-2 mRNA in T cells from T. cruzi-infected mice is not due to an increased degradation of the mRNA suggests a repression of the IL-2 gene at the transcriptional level. In this study,we have measured the level of nuclear factors that bind to specific sites on the IL-2 enhancer. Splenocytes and splenic T cells from acutely infected mice show a marked decrease in the level of AP-1, and a modest decrease in the level of NF-κB and nuclear factor of activated T cells (NF-AT). DNA-binding activity of Oct-1 was least affected in T cells from infected mice. Although the basal level of AP-1 activity is comparable in cells from uninfected and infected mice, mitogen-induced AP-1 activation is absent in the cells from T. cruzi-infected mice. Sodium deoxycholate treatment slightly enhances NF-κB-binding activity in splenocyte nuclear and whole-cell extracts from infected mice, suggesting that a blockage of the activation of NF-κB is only partially responsible for the decrease in the level of NF-κB in T cells from T. cruzi-infected mice. These data identify the molecular basis of IL-2 gene regulation in T. cruzi infection and suggest that T cells are anergized as a result of the infection.

Original languageEnglish (US)
Pages (from-to)16-23
Number of pages8
JournalEuropean Journal of Immunology
Volume24
Issue number1
StatePublished - Jan 1994
Externally publishedYes

Fingerprint

Trypanosoma cruzi
Interleukin-2
Infection
T-Lymphocytes
Transcription Factor AP-1
NFATC Transcription Factors
Deoxycholic Acid
Chagas Disease
RNA Stability
Lymphocyte Activation
Cell Extracts
Mitogens
Genes
Parasites
Gene Expression
Messenger RNA
DNA

Keywords

  • Interleukin-2 gene
  • Nuclear factors
  • Suppression
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Immunology

Cite this

Trypanosoma cruzi infection suppresses nuclear factors that bind to specific sites on the interleukin-2 enhancer. / Soong, Lynn; Tarleton, Rick L.

In: European Journal of Immunology, Vol. 24, No. 1, 01.1994, p. 16-23.

Research output: Contribution to journalArticle

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