Tube and column agglutination technology for autocontrol testing

J. E. Courtney, J. L. Vincent, A. J. Indrikovs

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of positive autocontrols in the ID Micro Typing System™ (gel) and the Gamma ReACT™ (REACT). The study encompassed a total of 1021 randomly selected samples from patients and 95 samples from donors collected during 1 month. The autocontrol testing was carried out according to the manufacturer's instructions for the column agglutination tests. The tube method was carried out using low-ionic-strength solution (LISS). The direct antiglobulin test (DAT) was performed using the tube method, and further investigated with elution studies if warranted. Seventy-nine patient's samples (7.74%) had a positive autocontrol: the gel test, 72 (91.13%); ReACT, 21 (26.58%) and the tube method, 27 (34.18%). Of the 79 positive autocontrols, 44 samples had a negative DAT. Of the samples with positive DAT results, only one possessed a clinically significant antibody, anti-D. Moreover, the same sample also tested positive in all three methods. Column agglutination techniques have increased sensitivity for a positive autocontrol beyond the conventional tube method. However, ReACT and gel tests differ significantly in their frequency of positives. Investigation of the significance of a positive autocontrol in column agglutination technology when the conventional tube method is also positive is suggested.

Original languageEnglish (US)
Pages (from-to)50-52
Number of pages3
Issue number2
StatePublished - 2001
Externally publishedYes


  • Autocontrol testing
  • ID Micro Typing System™
  • ReACT™

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology


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