Tumor exosomes inhibit differentiation of bone marrow dendritic cells

Shaohua Yu, Cunren Liu, Kaihong Su, Jianhua Wang, Yuelong Liu, Liming Zhang, Chuanyu Li, Yingzi Cong, Robert Kimberly, William E. Grizzle, Carla Falkson, Huang Ge Zhang

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

The production of exosomes by tumor cells has been implicated in tumor-associated immune suppression. In this study, we show that, in mice, exosomes produced by TS/A murine mammary tumor cells target CD11b+ myeloid precursors in the bone marrow (BM) in vivo, and that this is associated with an accumulation of myeloid precursors in the spleen. Moreover, we demonstrate that TS/A exosomes block the differentiation of murine myeloid precursor cells into dendritic cells (DC) in vitro. Addition of tumor exosomes at day 0 led to a significant block of differentiation into DC, whereas addition at later time points was less effective. Similarly, exosomes produced by human breast tumor cells inhibited the differentiation of human monocytes in vitro. The levels of IL-6 and phosphorylated Stat3 were elevated 12 h after the tumor exosome stimulation of murine myeloid precursors, and tumor exosomes were less effective in inhibiting differentiation of BM cells isolated from IL-6 knockout mice. Addition of a rIL-6 to the IL-6 knockout BM cell culture restored the tumor exosome-mediated inhibition of DC differentiation. These data suggest that tumor exosome-mediated induction of IL-6 plays a role in blocking BM DC differentiation.

Original languageEnglish (US)
Pages (from-to)6867-6875
Number of pages9
JournalJournal of Immunology
Volume178
Issue number11
StatePublished - Jun 1 2007
Externally publishedYes

Fingerprint

Exosomes
Bone Marrow Cells
Dendritic Cells
Neoplasms
Interleukin-6
Cell Differentiation
Breast Neoplasms
Myeloid Cells
Knockout Mice
Monocytes
Spleen
Cell Culture Techniques
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Yu, S., Liu, C., Su, K., Wang, J., Liu, Y., Zhang, L., ... Zhang, H. G. (2007). Tumor exosomes inhibit differentiation of bone marrow dendritic cells. Journal of Immunology, 178(11), 6867-6875.

Tumor exosomes inhibit differentiation of bone marrow dendritic cells. / Yu, Shaohua; Liu, Cunren; Su, Kaihong; Wang, Jianhua; Liu, Yuelong; Zhang, Liming; Li, Chuanyu; Cong, Yingzi; Kimberly, Robert; Grizzle, William E.; Falkson, Carla; Zhang, Huang Ge.

In: Journal of Immunology, Vol. 178, No. 11, 01.06.2007, p. 6867-6875.

Research output: Contribution to journalArticle

Yu, S, Liu, C, Su, K, Wang, J, Liu, Y, Zhang, L, Li, C, Cong, Y, Kimberly, R, Grizzle, WE, Falkson, C & Zhang, HG 2007, 'Tumor exosomes inhibit differentiation of bone marrow dendritic cells', Journal of Immunology, vol. 178, no. 11, pp. 6867-6875.
Yu S, Liu C, Su K, Wang J, Liu Y, Zhang L et al. Tumor exosomes inhibit differentiation of bone marrow dendritic cells. Journal of Immunology. 2007 Jun 1;178(11):6867-6875.
Yu, Shaohua ; Liu, Cunren ; Su, Kaihong ; Wang, Jianhua ; Liu, Yuelong ; Zhang, Liming ; Li, Chuanyu ; Cong, Yingzi ; Kimberly, Robert ; Grizzle, William E. ; Falkson, Carla ; Zhang, Huang Ge. / Tumor exosomes inhibit differentiation of bone marrow dendritic cells. In: Journal of Immunology. 2007 ; Vol. 178, No. 11. pp. 6867-6875.
@article{de851a2e3ee247678435de420620b854,
title = "Tumor exosomes inhibit differentiation of bone marrow dendritic cells",
abstract = "The production of exosomes by tumor cells has been implicated in tumor-associated immune suppression. In this study, we show that, in mice, exosomes produced by TS/A murine mammary tumor cells target CD11b+ myeloid precursors in the bone marrow (BM) in vivo, and that this is associated with an accumulation of myeloid precursors in the spleen. Moreover, we demonstrate that TS/A exosomes block the differentiation of murine myeloid precursor cells into dendritic cells (DC) in vitro. Addition of tumor exosomes at day 0 led to a significant block of differentiation into DC, whereas addition at later time points was less effective. Similarly, exosomes produced by human breast tumor cells inhibited the differentiation of human monocytes in vitro. The levels of IL-6 and phosphorylated Stat3 were elevated 12 h after the tumor exosome stimulation of murine myeloid precursors, and tumor exosomes were less effective in inhibiting differentiation of BM cells isolated from IL-6 knockout mice. Addition of a rIL-6 to the IL-6 knockout BM cell culture restored the tumor exosome-mediated inhibition of DC differentiation. These data suggest that tumor exosome-mediated induction of IL-6 plays a role in blocking BM DC differentiation.",
author = "Shaohua Yu and Cunren Liu and Kaihong Su and Jianhua Wang and Yuelong Liu and Liming Zhang and Chuanyu Li and Yingzi Cong and Robert Kimberly and Grizzle, {William E.} and Carla Falkson and Zhang, {Huang Ge}",
year = "2007",
month = "6",
day = "1",
language = "English (US)",
volume = "178",
pages = "6867--6875",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - Tumor exosomes inhibit differentiation of bone marrow dendritic cells

AU - Yu, Shaohua

AU - Liu, Cunren

AU - Su, Kaihong

AU - Wang, Jianhua

AU - Liu, Yuelong

AU - Zhang, Liming

AU - Li, Chuanyu

AU - Cong, Yingzi

AU - Kimberly, Robert

AU - Grizzle, William E.

AU - Falkson, Carla

AU - Zhang, Huang Ge

PY - 2007/6/1

Y1 - 2007/6/1

N2 - The production of exosomes by tumor cells has been implicated in tumor-associated immune suppression. In this study, we show that, in mice, exosomes produced by TS/A murine mammary tumor cells target CD11b+ myeloid precursors in the bone marrow (BM) in vivo, and that this is associated with an accumulation of myeloid precursors in the spleen. Moreover, we demonstrate that TS/A exosomes block the differentiation of murine myeloid precursor cells into dendritic cells (DC) in vitro. Addition of tumor exosomes at day 0 led to a significant block of differentiation into DC, whereas addition at later time points was less effective. Similarly, exosomes produced by human breast tumor cells inhibited the differentiation of human monocytes in vitro. The levels of IL-6 and phosphorylated Stat3 were elevated 12 h after the tumor exosome stimulation of murine myeloid precursors, and tumor exosomes were less effective in inhibiting differentiation of BM cells isolated from IL-6 knockout mice. Addition of a rIL-6 to the IL-6 knockout BM cell culture restored the tumor exosome-mediated inhibition of DC differentiation. These data suggest that tumor exosome-mediated induction of IL-6 plays a role in blocking BM DC differentiation.

AB - The production of exosomes by tumor cells has been implicated in tumor-associated immune suppression. In this study, we show that, in mice, exosomes produced by TS/A murine mammary tumor cells target CD11b+ myeloid precursors in the bone marrow (BM) in vivo, and that this is associated with an accumulation of myeloid precursors in the spleen. Moreover, we demonstrate that TS/A exosomes block the differentiation of murine myeloid precursor cells into dendritic cells (DC) in vitro. Addition of tumor exosomes at day 0 led to a significant block of differentiation into DC, whereas addition at later time points was less effective. Similarly, exosomes produced by human breast tumor cells inhibited the differentiation of human monocytes in vitro. The levels of IL-6 and phosphorylated Stat3 were elevated 12 h after the tumor exosome stimulation of murine myeloid precursors, and tumor exosomes were less effective in inhibiting differentiation of BM cells isolated from IL-6 knockout mice. Addition of a rIL-6 to the IL-6 knockout BM cell culture restored the tumor exosome-mediated inhibition of DC differentiation. These data suggest that tumor exosome-mediated induction of IL-6 plays a role in blocking BM DC differentiation.

UR - http://www.scopus.com/inward/record.url?scp=34249792918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249792918&partnerID=8YFLogxK

M3 - Article

VL - 178

SP - 6867

EP - 6875

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -