Tumor necrosis factor-α in human milk

H. Elizabeth Rudloff, Frank C. Schmalstieg, Akram A. Mushtaha, Kimberly H. Palkowetz, Stephen K. Liu, Armond S. Goldman

Research output: Contribution to journalArticle

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Abstract

We previously demonstrated that certain biologic activities in human milk were partially blocked by antibodies directed against human tumor necrosis factor-a (TNF-α). In this study, immunochemical methods were used to verify the presence of TNF-α in human milk obtained during the first few days of lactation. Gel filtration revealed the presence of TNF-α by RIA in molecular weight fractions between 80 and 195 kD. TNF-α could not be detected consistently by conventional Western blotting or cytotoxic assays. Although immunoreactive bands were detected by a Western blot-125I protein A technique in TNF-α-positive fractions from gel filtration, those bands proved to be nonspecific. TNF-α in milk was reliably quantified by the competitive RIA. Those studies revealed that the concentrations of TNF-α in milk were 620 ± 183 pg/mL. Although RNA to TNF-α was detected in milk leukocytes by Northern blotting, little TNF-α was found in those cells before or after stimulation with N-formyl-/-methionyl-/-leucyl-/-phenylalanine or 4β-phorbol-12β-my-ristate-13a-acetate. The origin of this cytokine in human milk remains unclear. Nevertheless, this study suggests that TNF-α is present in early human milk in sufficient quantities to exert possible biologic effects upon the mammary gland of the mother or the immune system of the infant.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalPediatric Research
Volume31
Issue number1
StatePublished - 1992

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Human Milk
Tumor Necrosis Factor-alpha
Milk
Gel Chromatography
Western Blotting
N-Formylmethionine Leucyl-Phenylalanine
Staphylococcal Protein A
Human Mammary Glands
Lactation
Northern Blotting
Immune System
Acetates
Leukocytes
Molecular Weight
Mothers
RNA
Cytokines
Antibodies

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Rudloff, H. E., Schmalstieg, F. C., Mushtaha, A. A., Palkowetz, K. H., Liu, S. K., & Goldman, A. S. (1992). Tumor necrosis factor-α in human milk. Pediatric Research, 31(1), 29-33.

Tumor necrosis factor-α in human milk. / Rudloff, H. Elizabeth; Schmalstieg, Frank C.; Mushtaha, Akram A.; Palkowetz, Kimberly H.; Liu, Stephen K.; Goldman, Armond S.

In: Pediatric Research, Vol. 31, No. 1, 1992, p. 29-33.

Research output: Contribution to journalArticle

Rudloff, HE, Schmalstieg, FC, Mushtaha, AA, Palkowetz, KH, Liu, SK & Goldman, AS 1992, 'Tumor necrosis factor-α in human milk', Pediatric Research, vol. 31, no. 1, pp. 29-33.
Rudloff HE, Schmalstieg FC, Mushtaha AA, Palkowetz KH, Liu SK, Goldman AS. Tumor necrosis factor-α in human milk. Pediatric Research. 1992;31(1):29-33.
Rudloff, H. Elizabeth ; Schmalstieg, Frank C. ; Mushtaha, Akram A. ; Palkowetz, Kimberly H. ; Liu, Stephen K. ; Goldman, Armond S. / Tumor necrosis factor-α in human milk. In: Pediatric Research. 1992 ; Vol. 31, No. 1. pp. 29-33.
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