Tunneling nanotubes (TNT) mediate long-range gap junctional communication

Implications for HIV cell to cell spread

George Okafo, Lisa Prevedel, Eliseo Eugenin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cell-to-cell communication is essen for the development of multicellular systems and is coordinated by soluble factors, exosomes, gap junction (GJ) channels, and the recently described tunneling nanotubes (TNTs). We and others have demonstrated that TNT-like structures are mostly present during pathogenic conditions, including HIV infection. However, the nature, function, and communication properties of TNTs are still poorly understood. In this manuscript, we demonstrate that TNTs induced by HIV infection have functional GJs at the ends of their membrane extensions and that TNTs mediate long-range GJ communication during HIV infection. Blocking or reducing GJ communication during HIV infection resulted in aberrant TNT cell-to-cell contact, compromising HIV spread and replication. Thus, TNTs and associated GJs are required for the efficient cell-to-cell communication and viral spread. Our data indicate that targeting TNTs/GJs may provide new therapeutic opportunities for the treatment of HIV.

Original languageEnglish (US)
Article number16660
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

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Nanotubes
HIV
HIV Infections
Gap Junctions
Cell Communication
Exosomes
Membranes

ASJC Scopus subject areas

  • General

Cite this

Tunneling nanotubes (TNT) mediate long-range gap junctional communication : Implications for HIV cell to cell spread. / Okafo, George; Prevedel, Lisa; Eugenin, Eliseo.

In: Scientific Reports, Vol. 7, No. 1, 16660, 01.12.2017.

Research output: Contribution to journalArticle

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