Turning the 10-23 DNAzyme on and off with light

Julia L. Richards; Garry K. Seward; Yu Hsiu Wang; Ivan J. Dmochowski

doi:10.1002/cbic.200900702

Turning the 10-23 DNAzyme on and off with light

Julia L. Richards, Garry K. Seward, Yu Hsiu Wang, Ivan J. Dmochowski

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

(Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

Original language	English (US)
Pages (from-to)	320-324
Number of pages	5
Journal	ChemBioChem
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/cbic.200900702
State	Published - Feb 15 2010
Externally published	Yes

Keywords

Cage compounds
DNAzymes
Light activation
Oligonucleotides
Photoregulation

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

Access to Document

10.1002/cbic.200900702

Cite this

@article{03f6223ed11c4acaba9a695c9a835106,

title = "Turning the 10-23 DNAzyme on and off with light",

abstract = "(Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched {"}on{"} or {"}off{"} with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained {"}off{"} until UV irradiation restored activity.",

keywords = "Cage compounds, DNAzymes, Light activation, Oligonucleotides, Photoregulation",

author = "Richards, {Julia L.} and Seward, {Garry K.} and Wang, {Yu Hsiu} and Dmochowski, {Ivan J.}",

year = "2010",

month = feb,

day = "15",

doi = "10.1002/cbic.200900702",

language = "English (US)",

volume = "11",

pages = "320--324",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Turning the 10-23 DNAzyme on and off with light

AU - Richards, Julia L.

AU - Seward, Garry K.

AU - Wang, Yu Hsiu

AU - Dmochowski, Ivan J.

PY - 2010/2/15

Y1 - 2010/2/15

N2 - (Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

AB - (Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

KW - Cage compounds

KW - DNAzymes

KW - Light activation

KW - Oligonucleotides

KW - Photoregulation

UR - http://www.scopus.com/inward/record.url?scp=76649118461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76649118461&partnerID=8YFLogxK

U2 - 10.1002/cbic.200900702

DO - 10.1002/cbic.200900702

M3 - Article

C2 - 20077457

AN - SCOPUS:76649118461

SN - 1439-4227

VL - 11

SP - 320

EP - 324

JO - ChemBioChem

JF - ChemBioChem

IS - 3

ER -

Turning the 10-23 DNAzyme on and off with light

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this