Turning the 10-23 DNAzyme on and off with light

Julia L. Richards, Garry K. Seward, Yu Hsiu Wang, Ivan J. Dmochowski

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

(Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

Original languageEnglish (US)
Pages (from-to)320-324
Number of pages5
JournalChemBioChem
Volume11
Issue number3
DOIs
StatePublished - Feb 15 2010
Externally publishedYes

Keywords

  • Cage compounds
  • DNAzymes
  • Light activation
  • Oligonucleotides
  • Photoregulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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