Turning the 10-23 DNAzyme on and off with light

Julia L. Richards; Garry K. Seward; Yu Hsiu Wang; Ivan J. Dmochowski

doi:10.1002/cbic.200900702

Turning the 10-23 DNAzyme on and off with light

Julia L. Richards
, Garry K. Seward
, Yu Hsiu Wang
, Ivan J. Dmochowski

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

(Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

Original language	English (US)
Pages (from-to)	320-324
Number of pages	5
Journal	ChemBioChem
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/cbic.200900702
State	Published - Feb 15 2010
Externally published	Yes

Keywords

Cage compounds
DNAzymes
Light activation
Oligonucleotides
Photoregulation

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200900702

Cite this

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title = "Turning the 10-23 DNAzyme on and off with light",

abstract = "(Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched {"}on{"} or {"}off{"} with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained {"}off{"} until UV irradiation restored activity.",

keywords = "Cage compounds, DNAzymes, Light activation, Oligonucleotides, Photoregulation",

author = "Richards, \{Julia L.\} and Seward, \{Garry K.\} and Wang, \{Yu Hsiu\} and Dmochowski, \{Ivan J.\}",

year = "2010",

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doi = "10.1002/cbic.200900702",

language = "English (US)",

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TY - JOUR

T1 - Turning the 10-23 DNAzyme on and off with light

AU - Richards, Julia L.

AU - Seward, Garry K.

AU - Wang, Yu Hsiu

AU - Dmochowski, Ivan J.

PY - 2010/2/15

Y1 - 2010/2/15

N2 - (Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

AB - (Figure Presented) Spaced out. DNAzymes incorporating photocleavable spacers were switched "on" or "off" with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable spacers remained "off" until UV irradiation restored activity.

KW - Cage compounds

KW - DNAzymes

KW - Light activation

KW - Oligonucleotides

KW - Photoregulation

UR - https://www.scopus.com/pages/publications/76649118461

UR - https://www.scopus.com/pages/publications/76649118461#tab=citedBy

U2 - 10.1002/cbic.200900702

DO - 10.1002/cbic.200900702

M3 - Article

C2 - 20077457

AN - SCOPUS:76649118461

SN - 1439-4227

VL - 11

SP - 320

EP - 324

JO - ChemBioChem

JF - ChemBioChem

IS - 3

ER -

Turning the 10-23 DNAzyme on and off with light

Abstract

Keywords

ASJC Scopus subject areas

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