Turnover of abnormal proteins in Bacillus megaterium and Saccharomyces cerevisiae

differences between in vivo and in vitro degradation

Ashok Chopra, Marie Strnadová, Jiří Chaloupka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Degradation of abnormal proteins in Bacillus megaterium and Saccharomyces cerevisiae in vivo was compared with that in cell-free extracts. Protein degradation in vivo, when the cells were labelled with 14C-leucine during growth in the presence of ethionine, was affected by the concentration of the analogue used. Proteins synthesized in the presence of 0.2-1 mM ethionine were degraded most rapidly in both organisms. The proteolytic enzyme system of yeast degraded the analogue-containing proteins in vitro faster than the normal proteins. This holds also for proteins synthesized in the presence of 5 mM ethionine, whose degradation in vivo was impaired. The proteolytic system of B. megaterium, on the other hand, was unable in vitro to differentiate between normal and abnormal proteins. Denatured proteins underwent preferential degradation over normal and ethionine-containing proteins.

Original languageEnglish (US)
Pages (from-to)97-103
Number of pages7
JournalArchives of Microbiology
Volume145
Issue number1
DOIs
StatePublished - Jun 1986
Externally publishedYes

Fingerprint

Bacillus megaterium
Bacilli
Yeast
Saccharomyces cerevisiae
Ethionine
Degradation
Proteins
Proteolysis
In Vitro Techniques
Cell Extracts
Leucine
Peptide Hydrolases
Yeasts
Growth

Keywords

  • Abnormal protein
  • Bacillus megaterium
  • Ethionine
  • Protein degradation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Microbiology

Cite this

Turnover of abnormal proteins in Bacillus megaterium and Saccharomyces cerevisiae : differences between in vivo and in vitro degradation. / Chopra, Ashok; Strnadová, Marie; Chaloupka, Jiří.

In: Archives of Microbiology, Vol. 145, No. 1, 06.1986, p. 97-103.

Research output: Contribution to journalArticle

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