Twenty odd years of stretch-sensitive channels

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

After formation of the giga-seal, the membrane patch can be stimulated by hydrostatic or osmotic pressure gradients applied across the patch. This feature led to the discovery of stretch-sensitive or mechanosensitive (MS) channels, which are now known to be ubiquitously expressed in cells representative of all the living kingdoms. In addition to mechanosensation, MS channels have been implicated in many basic cell functions, including regulation of cell volume, shape, and motility. The successful cloning, overexpression, and crystallization of bacterial MS channel proteins combined with patch clamp and modeling studies have provided atomic insight into the working of these nanomachines. In particular, studies of MS channels have revealed new understanding of how the lipid bilayer modulates membrane protein function. Three major membrane protein families, transient receptor potential, 2 pore domain K+, and the epithelial Na+ channels, have been shown to form MS channels in animal cells, and their polymodal activation embrace fields far beyond mechanosensitivity. The discovery of new drugs highly selective for MS channels ("mechanopharmaceutics") and the demonstration of MS channel involvement in several major human diseases ("mechanochannelopathies") provide added motivation for devising new techniques and approaches for studying MS channels.

Original languageEnglish (US)
Pages (from-to)333-351
Number of pages19
JournalPflugers Archiv European Journal of Physiology
Volume453
Issue number3
DOIs
StatePublished - Dec 2006

Fingerprint

Membrane Proteins
Cells
Epithelial Sodium Channels
Lipid bilayers
Cloning
Clamping devices
Crystallization
Pressure gradient
Seals
Animals
Hydrostatic Pressure
Demonstrations
Cell Shape
Chemical activation
Osmotic Pressure
Lipid Bilayers
Drug Discovery
Membranes
Cell Size
Cell Movement

Keywords

  • Giga seal
  • Mechanosensitive channels
  • Mechanotransduction
  • Patch clamp
  • Transient receptor potential

ASJC Scopus subject areas

  • Physiology

Cite this

Twenty odd years of stretch-sensitive channels. / Hamill, Owen.

In: Pflugers Archiv European Journal of Physiology, Vol. 453, No. 3, 12.2006, p. 333-351.

Research output: Contribution to journalArticle

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