Abstract
After formation of the giga-seal, the membrane patch can be stimulated by hydrostatic or osmotic pressure gradients applied across the patch. This feature led to the discovery of stretch-sensitive or mechanosensitive (MS) channels, which are now known to be ubiquitously expressed in cells representative of all the living kingdoms. In addition to mechanosensation, MS channels have been implicated in many basic cell functions, including regulation of cell volume, shape, and motility. The successful cloning, overexpression, and crystallization of bacterial MS channel proteins combined with patch clamp and modeling studies have provided atomic insight into the working of these nanomachines. In particular, studies of MS channels have revealed new understanding of how the lipid bilayer modulates membrane protein function. Three major membrane protein families, transient receptor potential, 2 pore domain K+, and the epithelial Na+ channels, have been shown to form MS channels in animal cells, and their polymodal activation embrace fields far beyond mechanosensitivity. The discovery of new drugs highly selective for MS channels ("mechanopharmaceutics") and the demonstration of MS channel involvement in several major human diseases ("mechanochannelopathies") provide added motivation for devising new techniques and approaches for studying MS channels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 333-351 |
| Number of pages | 19 |
| Journal | Pflugers Archiv European Journal of Physiology |
| Volume | 453 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2006 |
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Keywords
- Giga seal
- Mechanosensitive channels
- Mechanotransduction
- Patch clamp
- Transient receptor potential
ASJC Scopus subject areas
- Physiology
Cite this
Twenty odd years of stretch-sensitive channels. / Hamill, Owen.
In: Pflugers Archiv European Journal of Physiology, Vol. 453, No. 3, 12.2006, p. 333-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Twenty odd years of stretch-sensitive channels
AU - Hamill, Owen
PY - 2006/12
Y1 - 2006/12
N2 - After formation of the giga-seal, the membrane patch can be stimulated by hydrostatic or osmotic pressure gradients applied across the patch. This feature led to the discovery of stretch-sensitive or mechanosensitive (MS) channels, which are now known to be ubiquitously expressed in cells representative of all the living kingdoms. In addition to mechanosensation, MS channels have been implicated in many basic cell functions, including regulation of cell volume, shape, and motility. The successful cloning, overexpression, and crystallization of bacterial MS channel proteins combined with patch clamp and modeling studies have provided atomic insight into the working of these nanomachines. In particular, studies of MS channels have revealed new understanding of how the lipid bilayer modulates membrane protein function. Three major membrane protein families, transient receptor potential, 2 pore domain K+, and the epithelial Na+ channels, have been shown to form MS channels in animal cells, and their polymodal activation embrace fields far beyond mechanosensitivity. The discovery of new drugs highly selective for MS channels ("mechanopharmaceutics") and the demonstration of MS channel involvement in several major human diseases ("mechanochannelopathies") provide added motivation for devising new techniques and approaches for studying MS channels.
AB - After formation of the giga-seal, the membrane patch can be stimulated by hydrostatic or osmotic pressure gradients applied across the patch. This feature led to the discovery of stretch-sensitive or mechanosensitive (MS) channels, which are now known to be ubiquitously expressed in cells representative of all the living kingdoms. In addition to mechanosensation, MS channels have been implicated in many basic cell functions, including regulation of cell volume, shape, and motility. The successful cloning, overexpression, and crystallization of bacterial MS channel proteins combined with patch clamp and modeling studies have provided atomic insight into the working of these nanomachines. In particular, studies of MS channels have revealed new understanding of how the lipid bilayer modulates membrane protein function. Three major membrane protein families, transient receptor potential, 2 pore domain K+, and the epithelial Na+ channels, have been shown to form MS channels in animal cells, and their polymodal activation embrace fields far beyond mechanosensitivity. The discovery of new drugs highly selective for MS channels ("mechanopharmaceutics") and the demonstration of MS channel involvement in several major human diseases ("mechanochannelopathies") provide added motivation for devising new techniques and approaches for studying MS channels.
KW - Giga seal
KW - Mechanosensitive channels
KW - Mechanotransduction
KW - Patch clamp
KW - Transient receptor potential
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UR - http://www.scopus.com/inward/citedby.url?scp=33751101567&partnerID=8YFLogxK
U2 - 10.1007/s00424-006-0131-0
DO - 10.1007/s00424-006-0131-0
M3 - Article
C2 - 17021800
AN - SCOPUS:33751101567
VL - 453
SP - 333
EP - 351
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
SN - 0031-6768
IS - 3
ER -