Two capa-genes are expressed in the neuroendocrine system of Rhodnius prolixus

Susanne Neupert, William Russell, David H. Russell, Reinhard Predel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

CAPA-peptides have been isolated from a broad range of insect species and are typical of the neurosecretory system of the abdominal ventral nerve cord. In Rhodnius prolixus, which is known to transmit Chagas' disease, CAPA-peptides have potent antidiuretic effects. Recently, two capa-genes were isolated from this hemipteran insect; it was the first description of the occurrence of multiple capa-genes from a single insect species. The expression of peptides from one of these capa-genes was confirmed by mass spectrometry Paluzzi (2008) [10]. In this study, the abdominal neuroendocrine system of R. prolixus was screened for the occurrence of products of the second annotated capa-gene. Single nerve preparations of abdominal segmental nerves 1-3 of R. prolixus were analyzed by MALDI-TOF mass spectrometry and the products of both capa-genes were identified by tandem mass spectrometry. The co-occurrence of the different CAPA-peptides, including CAPA-precursor peptides, in all spectra from abdominal segmental nerves 1 to 3 suggests a common expression of both capa-genes in the same neuroendocrine cells. A comparison of mass spectra obtained from abdominal segmental nerves with those of the subesophageal ganglion (SEG) revealed an obvious differential processing of the CAPA-precursors within the CNS.

Original languageEnglish (US)
Pages (from-to)408-411
Number of pages4
JournalPeptides
Volume31
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Fingerprint

Rhodnius
Neurosecretory Systems
Genes
Peptides
Mass spectrometry
Insects
Mass Spectrometry
Antidiuretic Agents
Neuroendocrine Cells
Chagas Disease
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tandem Mass Spectrometry
Ganglia
Processing

Keywords

  • Differential processing
  • Hemiptera
  • MALDI-TOF mass spectrometry
  • Neuropeptides
  • Periviscerokinins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Two capa-genes are expressed in the neuroendocrine system of Rhodnius prolixus. / Neupert, Susanne; Russell, William; Russell, David H.; Predel, Reinhard.

In: Peptides, Vol. 31, No. 3, 03.2010, p. 408-411.

Research output: Contribution to journalArticle

Neupert, Susanne ; Russell, William ; Russell, David H. ; Predel, Reinhard. / Two capa-genes are expressed in the neuroendocrine system of Rhodnius prolixus. In: Peptides. 2010 ; Vol. 31, No. 3. pp. 408-411.
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