Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography

Alain Brisson, Anders Olofsson, Philippe Ringler, Marc Schmutz, Svetla Stoylova

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Electron crystallography constitutes a powerful new method for determining the struture of biological macromolecules. This method is best adapted to the study of ordered assemblies of macromolecules, and principally to two-dimensional (2-D) crystals of proteins. Obtaining protein 2-D crystals ordered at high resolution constitutes the major limiting step in the application of this approach. Considerable interest has been raised by the development of a rational method of 2-D crystallization based on the specific binding of proteins to planar lipid films. The applicability of this method is quasi-general in the case of soluble proteins. Its basic principles, together with examples taken from work in our group, are presented here.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalBiology of the Cell
Volume80
Issue number2-3
StatePublished - 1994
Externally publishedYes

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Crystallography
Crystallization
Electrons
Lipids
Proteins
Carrier Proteins

Keywords

  • 2-D crystals
  • electron crystallography
  • membrane-bound proteins
  • planar lipid films

ASJC Scopus subject areas

  • Cell Biology

Cite this

Brisson, A., Olofsson, A., Ringler, P., Schmutz, M., & Stoylova, S. (1994). Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography. Biology of the Cell, 80(2-3), 221-228.

Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography. / Brisson, Alain; Olofsson, Anders; Ringler, Philippe; Schmutz, Marc; Stoylova, Svetla.

In: Biology of the Cell, Vol. 80, No. 2-3, 1994, p. 221-228.

Research output: Contribution to journalArticle

Brisson, A, Olofsson, A, Ringler, P, Schmutz, M & Stoylova, S 1994, 'Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography', Biology of the Cell, vol. 80, no. 2-3, pp. 221-228.
Brisson, Alain ; Olofsson, Anders ; Ringler, Philippe ; Schmutz, Marc ; Stoylova, Svetla. / Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography. In: Biology of the Cell. 1994 ; Vol. 80, No. 2-3. pp. 221-228.
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