We have made a comparison between plasma and endothelial cell fibronectin, since these cells are in intimate contact with plasma in vivo. Cellular and secreted fibronectins were purified from cloned lines of adult bovine aortic endothelial cells, and compared to purified bovine plasma fibronectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional peptide mapping. When unreduced, all three fibronectins migrated on gels as single bands with Mr 440,000. After reduction, cellular and secreted fibronectins migrated on gels as single bands with Mr 220,000, but plasma fibronectin migrated as two bands with Mr 220,000 and 210,000. All three fibronectins, including the two subunits of plasma fibronectin, had identical structures by peptide mapping analysis.
- adult bovine aortic endothelial
- sodium dodecyl sulfate
ASJC Scopus subject areas
- Molecular Biology