Two distinct modes of myosin assembly and dynamics during epithelial wound closure

Masako Tamada, Tomas D. Perez, W. James Nelson, Michael Sheetz

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin-Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalJournal of Cell Biology
Volume176
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

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Myosins
rho-Associated Kinases
Wounds and Injuries
Myosin Type II
Actomyosin
Tight Junctions
Myosin Light Chains
Madin Darby Canine Kidney Cells
Membranes
Laser Therapy
Embryonic Development
Actins
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Two distinct modes of myosin assembly and dynamics during epithelial wound closure. / Tamada, Masako; Perez, Tomas D.; Nelson, W. James; Sheetz, Michael.

In: Journal of Cell Biology, Vol. 176, No. 1, 01.01.2007, p. 27-33.

Research output: Contribution to journalArticle

Tamada, Masako ; Perez, Tomas D. ; Nelson, W. James ; Sheetz, Michael. / Two distinct modes of myosin assembly and dynamics during epithelial wound closure. In: Journal of Cell Biology. 2007 ; Vol. 176, No. 1. pp. 27-33.
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