Two mammalian UNC-45 isoforms are related to distinct cytoskeletal and muscle-specific functions

Maureen G. Price, Megan L. Landsverk, Jose M. Barral, Henry F. Epstein

    Research output: Contribution to journalReview article

    94 Scopus citations

    Abstract

    Previous studies have shown that the UNC-45 protein of C. elegans is required for normal thick filament assembly, binds Hsp90 and the myosin head, and shows molecular chaperone activity. We report here that mice and humans each have two genes that are located on different chromosomes, encode distinct UNC-45-like protein isoforms, and are expressed either in multiple tissues or only in cardiac and skeletal muscles. Their expression is regulated during muscle differentiation in vitro, with the striated muscle isoform mRNA appearing during myoblast fusion. Antisense experiments in C2C12 skeletal myogenic cells demonstrate that decreasing the general cell isoform mRNA reduces proliferation and fusion, while decreasing the striated muscle isoform mRNA affects fusion and sarcomere organization. These results suggest that the general cell UNC-45 isoform may have primarily cytoskeletal functions and that the striated muscle UNC-45 isoform may be restricted to roles in muscle-specific differentiation.

    Original languageEnglish (US)
    Pages (from-to)4013-4023
    Number of pages11
    JournalJournal of Cell Science
    Volume115
    Issue number21
    DOIs
    StatePublished - Nov 1 2002

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    Keywords

    • Chaperone
    • Muscle differentiation
    • Myosin
    • Proliferation
    • UNC-45

    ASJC Scopus subject areas

    • Cell Biology

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