Two-photon autofluorescence spectroscopy of oral mucosa tissue

Kert Edward, Tuya Shilagard, Suimin Qiu, Gracie Vargasa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The survival rate for individuals diagnosed with oral cancer is correlated with the stage of detection. Thus the development of novel techniques for the earliest possible detection of malignancies is of critical importance. Single photon (1P) autofluorescence spectroscopy has proven to be a powerful diagnostic tool in this regard, but 2P (two photon) spectroscopy remains essentially unexplored. In this investigation, a spectroscopic system was incorporated into a custom-built 2P laser scanning microscope. Oral cancer was induced in the buccal pouch of Syrian Golden hamsters by tri-weekly topical application of 9,10-dimethyl-1,2-benzanthracene (DMBA).Three separated sites where investigated in each hamster at four excitation wavelengths from 780 nm to 890 nm. A Total of 8 hamsters were investigated (4 normal and 4 DMBA treated). All investigated sites were imaged via 2p imaging, marked for biopsy, processed for histology and H&E staining, and graded by a pathologist. The in vivo emission spectrum for normal, mild/high grade dysplasia and squamous cell carcinoma is presented. It is shown that the hamsters with various stages of dysplasia are characterized by spectral differences as a function of depth and excitation wavelength, compared to normal hamsters.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7903
DOIs
StatePublished - 2011
EventMultiphoton Microscopy in the Biomedical Sciences XI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XI
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Fingerprint

hamsters
Mouth Mucosa
Photons
Cricetinae
Spectrum Analysis
Spectroscopy
Tissue
Wavelength
9,10-Dimethyl-1,2-benzanthracene
Histology
Biopsy
Mouth Neoplasms
photons
spectroscopy
cancer
Microscopes
Scanning
Imaging techniques
Lasers
Cheek

Keywords

  • Multiphoton microscopy
  • Oral carcinoma
  • Oral mucosa
  • Spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Edward, K., Shilagard, T., Qiu, S., & Vargasa, G. (2011). Two-photon autofluorescence spectroscopy of oral mucosa tissue. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7903). [79031Q] https://doi.org/10.1117/12.875049

Two-photon autofluorescence spectroscopy of oral mucosa tissue. / Edward, Kert; Shilagard, Tuya; Qiu, Suimin; Vargasa, Gracie.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7903 2011. 79031Q.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Edward, K, Shilagard, T, Qiu, S & Vargasa, G 2011, Two-photon autofluorescence spectroscopy of oral mucosa tissue. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7903, 79031Q, Multiphoton Microscopy in the Biomedical Sciences XI, San Francisco, CA, United States, 1/23/11. https://doi.org/10.1117/12.875049
Edward K, Shilagard T, Qiu S, Vargasa G. Two-photon autofluorescence spectroscopy of oral mucosa tissue. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7903. 2011. 79031Q https://doi.org/10.1117/12.875049
Edward, Kert ; Shilagard, Tuya ; Qiu, Suimin ; Vargasa, Gracie. / Two-photon autofluorescence spectroscopy of oral mucosa tissue. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7903 2011.
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