Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin

Guoying Jiang, Grégory Giannone, David R. Critchley, Emiko Fukumoto, Michael Sheetz

Research output: Contribution to journalArticle

321 Citations (Scopus)

Abstract

Mechanical forces on matrix-integrin-cytoskeleton linkages are crucial for cell viability, morphology and organ function. The production of force depends on the molecular connections from extracellular-matrix-integrin complexes to the cytoskeleton. The minimal matrix complex causing integrin-cytoskeleton connections is a trimer of fibronectin's integrin-binding domain FNIII7-10 (ref. 4). Here we report a specific, molecular slip bond that was broken repeatedly by a force of 2 pN at the cellular loading rate of 60 nm s-1; this occurred with single trimer beads but not with monomer. Talin1, which binds to both integrins and actin filaments in vitro, is required for the 2-pN slip bond and rapid cytoskeleton binding. Further, inhibition of fibronectin binding to αvβ3 and deletion of β3 markedly decreases the 2-pN force peak. We suggest that talin1 initially forms a molecular slip bond between closely packed fibronectin-integrin complexes and the actin cytoskeleton, which can apply a low level of force to fibronectin until many bonds form or a signal is received to activate a force response.

Original languageEnglish (US)
Pages (from-to)334-337
Number of pages4
JournalNature
Volume424
Issue number6946
DOIs
StatePublished - Jul 17 2003
Externally publishedYes

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Talin
Cytoskeleton
Fibronectins
Integrins
Actin Cytoskeleton
Extracellular Matrix
Cell Survival

ASJC Scopus subject areas

  • General

Cite this

Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin. / Jiang, Guoying; Giannone, Grégory; Critchley, David R.; Fukumoto, Emiko; Sheetz, Michael.

In: Nature, Vol. 424, No. 6946, 17.07.2003, p. 334-337.

Research output: Contribution to journalArticle

Jiang, G, Giannone, G, Critchley, DR, Fukumoto, E & Sheetz, M 2003, 'Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin', Nature, vol. 424, no. 6946, pp. 334-337. https://doi.org/10.1038/nature01805
Jiang, Guoying ; Giannone, Grégory ; Critchley, David R. ; Fukumoto, Emiko ; Sheetz, Michael. / Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin. In: Nature. 2003 ; Vol. 424, No. 6946. pp. 334-337.
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