Type I interferon-dependent and -independent expression of tripartite motif proteins in immune cells

Ricardo Rajsbaum Gorodezky, Jonathan P. Stoye, Anne O'Garra

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The tripartite motif (TRIM) proteins are important in a variety of cellular functions additional to anti-viral activity. We systematically analysed mRNA expression of representative TRIM molecules in mouse macrophages, myeloid and plasmacytoid dendritic cells, and a selection of CD4+ T cell subsets. We defined four clusters of TRIM genes based on their selective expression in these cells. The first group of TRIM genes was preferentially expressed in CD4+ T cells and contained the COS-FN3 motif previously shown to be involved in protein interactions. Additional TRIM genes were identified that showed up-regulation in macrophages and dendritic cells upon influenza virus infection in a type I IFN-dependent manner, suggesting that they have anti-viral activity. In support of this notion, a subset of these TRIM molecules mapped to mouse chromosome 7, syntenic to human chromosome 11, where TRIM family members such as TRIM5, shown to have anti-viral activity, are localized. A distinct group of TRIM was constitutively expressed in plasmacytoid dendritic cells independently of viral infection or signalling through the type I IFN receptor. Our findings on expression and regulation of TRIM genes in cells of the immune system that have different effector functions in innate and adaptive immune responses, may provide leads for determining functions of this diverse family of molecules.

Original languageEnglish (US)
Pages (from-to)619-630
Number of pages12
JournalEuropean Journal of Immunology
Volume38
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Interferon Type I
Dendritic Cells
Virus Diseases
Genes
Macrophages
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 7
Human Chromosomes
T-Lymphocyte Subsets
Adaptive Immunity
Orthomyxoviridae
Innate Immunity
Immune System
Up-Regulation
T-Lymphocytes
Messenger RNA
Tripartite Motif Proteins
Proteins

Keywords

  • CD4 T cells
  • Dendritic cells
  • Innate immunity
  • Interferon
  • Macrophages

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Type I interferon-dependent and -independent expression of tripartite motif proteins in immune cells. / Rajsbaum Gorodezky, Ricardo; Stoye, Jonathan P.; O'Garra, Anne.

In: European Journal of Immunology, Vol. 38, No. 3, 03.2008, p. 619-630.

Research output: Contribution to journalArticle

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