U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication

Mee Kian Poh, Guanghou Shui, Xuping Xie, Pei-Yong Shi, Markus R. Wenk, Feng Gu

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The level of cholesterol in host cells has been shown to affect viral infection. However, it is still not understood why this level of regulation is important for successful infection. We have shown in this study that dengue virus infection was affected when the cholesterol intake in infected cells was disrupted using a cholesterol transport inhibitor, U18666A. The antiviral effect was found to result from two events: retarded viral trafficking in the cholesterol-loaded late endosomes/lysosomes and suppressed de novo sterol biosynthesis in treated infected cells. We also observed an additive antiviral effect of U18666A with C75, a fatty acid synthase inhibitor, suggesting dengue virus relies on both the host cholesterol and fatty acid biosynthesis for successful replication.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalAntiviral Research
Volume93
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Fingerprint

Anticholesteremic Agents
Virus Internalization
Dengue Virus
Virus Replication
Cholesterol
Virus Diseases
Antiviral Agents
Fatty Acid Synthases
Endosomes
Sterols
Lysosomes
Fatty Acids
3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one
Infection

Keywords

  • Antiviral
  • Cholesterol
  • Dengue virus
  • Fatty acids
  • U18666A

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication. / Poh, Mee Kian; Shui, Guanghou; Xie, Xuping; Shi, Pei-Yong; Wenk, Markus R.; Gu, Feng.

In: Antiviral Research, Vol. 93, No. 1, 01.2012, p. 191-198.

Research output: Contribution to journalArticle

Poh, Mee Kian ; Shui, Guanghou ; Xie, Xuping ; Shi, Pei-Yong ; Wenk, Markus R. ; Gu, Feng. / U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication. In: Antiviral Research. 2012 ; Vol. 93, No. 1. pp. 191-198.
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