UAP56 is an important regulator of protein synthesis and growth in cardiomyocytes

Abha Sahni, Nadan Wang, Jeffrey D. Alexis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

UAP56, an ATP dependent RNA helicase that also has ATPase activity, is a DExD/H box protein that is phylogenetically grouped with the eukaryotic initiation factor eIF4A, the prototypical member of the DExD/H box family of helicases. UAP56, also known as BAT1, is an essential RNA splicing factor required for spliceosome assembly and mRNA export but its role in protein synthesis is not known. Here we demonstrate that UAP56 regulates protein synthesis and growth in cardiomyocytes. We found that wild-type (WT) UAP56 increased serum induced protein synthesis in HeLa cells. UAP56 mutants lacking ATPase and/or helicase activity inhibited protein synthesis compared with WT UAP56, suggesting that the ATPase and RNA helicase activity of UAP56 is important for protein synthesis. UAP56 siRNA inhibited phenylephrine (PE) induced protein synthesis in cardiomyocytes and inhibited PE induced cardiomyocyte hypertrophy. Our data demonstrate that UAP56 is an important regulator of protein synthesis and plays an important role in the regulation of cardiomyocyte growth.

Original languageEnglish (US)
Pages (from-to)106-110
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number1
DOIs
StatePublished - Feb 26 2010
Externally publishedYes

Fingerprint

Cardiac Myocytes
Growth
Proteins
RNA Helicases
Adenosine Triphosphatases
Phenylephrine
Spliceosomes
Eukaryotic Initiation Factors
HeLa Cells
Hypertrophy
Small Interfering RNA
Blood Proteins
Adenosine Triphosphate
Messenger RNA
RNA

Keywords

  • Cardiac hypertrophy
  • Growth
  • Helicase
  • Protein synthesis
  • UAP56

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

UAP56 is an important regulator of protein synthesis and growth in cardiomyocytes. / Sahni, Abha; Wang, Nadan; Alexis, Jeffrey D.

In: Biochemical and Biophysical Research Communications, Vol. 393, No. 1, 26.02.2010, p. 106-110.

Research output: Contribution to journalArticle

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