Ultrasound improves tissue perfusion in ischemic tissue through a nitric oxide dependent mechanism

Valentina N. Suchkova, Raymond B. Baggs, Sanjeev Sahni, Charles W. Francis

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Ultrasound accelerates enzymatic fibrinolysis in vitro and in animal models and may be used as an adjunct to thrombolytic therapy. Ultrasound can also affect vascular tone directly, and we have now investigated the effect of ultrasound on tissue perfusion in a rabbit model of acute muscle ischemia to characterize the magnitude and temporal course of vasodilation and determine its mechanism. After ligation of the femoral artery of rabbits, tissue perfusion in the gracilis muscle as determined using a laser Doppler probe declined by 53% from 13.7 ± 0.3 U to 6.4 ± 0.2 U. The tissue became acidotic as pH declined from normal to 7.05 ± 0.2. Application of 40 kHz ultrasound at intensities from 0.25 to 0.75 W/cm2 progressively improved perfusion over 60 min and reversed acidosis, but these effects were both completely blocked by pre-treatment with the nitric oxide synthase inhibitor L-NAME. Nitric oxide synthase activity in muscle was measured using an assay based on the conversion of radiolabeled L-arginine to L-citrulline and demonstrated an increase of 3.6-fold following ultrasound exposure. This effect was greatest at locations close to the transducer and declined progressively away from it. Histologic examination showed greater capillary circumference in ultrasound exposed muscle compared to unexposed tissue with no other histologic changes. We conclude that the application of 40 kHz at low intensity improves perfusion and reverses acidosis in acutely ischemic muscle through a nitric oxide dependent mechanism.

Original languageEnglish (US)
Pages (from-to)865-870
Number of pages6
JournalThrombosis and Haemostasis
Volume88
Issue number5
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Nitric Oxide
Perfusion
Muscles
Acidosis
Nitric Oxide Synthase
Rabbits
Citrulline
Thrombolytic Therapy
NG-Nitroarginine Methyl Ester
Fibrinolysis
Femoral Artery
Transducers
Vasodilation
Ligation
Blood Vessels
Arginine
Lasers
Ischemia
Animal Models
Therapeutics

Keywords

  • Ischemia
  • Nitric oxide
  • Nitric oxide synthase
  • Ultrasound

ASJC Scopus subject areas

  • Hematology

Cite this

Ultrasound improves tissue perfusion in ischemic tissue through a nitric oxide dependent mechanism. / Suchkova, Valentina N.; Baggs, Raymond B.; Sahni, Sanjeev; Francis, Charles W.

In: Thrombosis and Haemostasis, Vol. 88, No. 5, 01.11.2002, p. 865-870.

Research output: Contribution to journalArticle

Suchkova, Valentina N. ; Baggs, Raymond B. ; Sahni, Sanjeev ; Francis, Charles W. / Ultrasound improves tissue perfusion in ischemic tissue through a nitric oxide dependent mechanism. In: Thrombosis and Haemostasis. 2002 ; Vol. 88, No. 5. pp. 865-870.
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