Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region

Ana L M Wanzeller, José A P Diniz, Maria L C Gomes, Ana C R Cruz, Manoel C P Soares, Wanderley De Souza, Amélia P A Travassos Da Rosa, Pedro F C Vasconcelos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.

Original languageEnglish (US)
Pages (from-to)307-311
Number of pages5
JournalMemorias do Instituto Oswaldo Cruz
Volume97
Issue number3
StatePublished - Apr 2002

Fingerprint

Orthobunyavirus
Viruses
Vero Cells
Virion
Bunyaviridae
Arboviruses
Virus Assembly
Complement Fixation Tests
Negative Staining
Viral Proteins
Infection
Brazil
Cell culture
Electron Microscopy
Liver
Electron microscopy
Cell Culture Techniques
Brain
Blood
Kinetics

Keywords

  • Amazon region
  • Arbovirus
  • Brazil
  • Characterization
  • Mojuí dos Campos virus
  • Protein kinetics
  • Ultrastructure

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Virology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Wanzeller, A. L. M., Diniz, J. A. P., Gomes, M. L. C., Cruz, A. C. R., Soares, M. C. P., De Souza, W., ... Vasconcelos, P. F. C. (2002). Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region. Memorias do Instituto Oswaldo Cruz, 97(3), 307-311.

Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region. / Wanzeller, Ana L M; Diniz, José A P; Gomes, Maria L C; Cruz, Ana C R; Soares, Manoel C P; De Souza, Wanderley; Travassos Da Rosa, Amélia P A; Vasconcelos, Pedro F C.

In: Memorias do Instituto Oswaldo Cruz, Vol. 97, No. 3, 04.2002, p. 307-311.

Research output: Contribution to journalArticle

Wanzeller, ALM, Diniz, JAP, Gomes, MLC, Cruz, ACR, Soares, MCP, De Souza, W, Travassos Da Rosa, APA & Vasconcelos, PFC 2002, 'Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region', Memorias do Instituto Oswaldo Cruz, vol. 97, no. 3, pp. 307-311.
Wanzeller, Ana L M ; Diniz, José A P ; Gomes, Maria L C ; Cruz, Ana C R ; Soares, Manoel C P ; De Souza, Wanderley ; Travassos Da Rosa, Amélia P A ; Vasconcelos, Pedro F C. / Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region. In: Memorias do Instituto Oswaldo Cruz. 2002 ; Vol. 97, No. 3. pp. 307-311.
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