Abstract
The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-311 |
| Number of pages | 5 |
| Journal | Memorias do Instituto Oswaldo Cruz |
| Volume | 97 |
| Issue number | 3 |
| State | Published - Apr 2002 |
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Keywords
- Amazon region
- Arbovirus
- Brazil
- Characterization
- Mojuí dos Campos virus
- Protein kinetics
- Ultrastructure
ASJC Scopus subject areas
- Clinical Biochemistry
- Immunology
- Virology
- Infectious Diseases
- Microbiology (medical)
Cite this
Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region. / Wanzeller, Ana L M; Diniz, José A P; Gomes, Maria L C; Cruz, Ana C R; Soares, Manoel C P; De Souza, Wanderley; Travassos Da Rosa, Amélia P A; Vasconcelos, Pedro F C.
In: Memorias do Instituto Oswaldo Cruz, Vol. 97, No. 3, 04.2002, p. 307-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrastructural, antigenic and physicochemical characterization of the mojuí dos campos (Bunyavirus) isolated from bat in the Brazilian Amazon Region
AU - Wanzeller, Ana L M
AU - Diniz, José A P
AU - Gomes, Maria L C
AU - Cruz, Ana C R
AU - Soares, Manoel C P
AU - De Souza, Wanderley
AU - Travassos Da Rosa, Amélia P A
AU - Vasconcelos, Pedro F C
PY - 2002/4
Y1 - 2002/4
N2 - The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.
AB - The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.
KW - Amazon region
KW - Arbovirus
KW - Brazil
KW - Characterization
KW - Mojuí dos Campos virus
KW - Protein kinetics
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=0036548836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036548836&partnerID=8YFLogxK
M3 - Article
VL - 97
SP - 307
EP - 311
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
SN - 0074-0276
IS - 3
ER -