Ultrastructural Characterization of SARS Coronavirus

Cynthia S. Goldsmith, Kathleen M. Tatti, Thomas Ksiazek, Pierre E. Rollin, James A. Comer, William W. Lee, Paul A. Rota, Bettina Bankamp, William J. Bellini, Sherif R. Zaki

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalEmerging Infectious Diseases
Volume10
Issue number2
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

Severe Acute Respiratory Syndrome
Coronavirus
Nucleocapsid
Disease Outbreaks
Electrons
Therapeutic Irrigation
Viral RNA
Viral Proteins
Virion
In Situ Hybridization
Pneumonia
Cytoplasm
Cell Culture Techniques
Viruses
Membranes
Infection

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Goldsmith, C. S., Tatti, K. M., Ksiazek, T., Rollin, P. E., Comer, J. A., Lee, W. W., ... Zaki, S. R. (2004). Ultrastructural Characterization of SARS Coronavirus. Emerging Infectious Diseases, 10(2), 320-326.

Ultrastructural Characterization of SARS Coronavirus. / Goldsmith, Cynthia S.; Tatti, Kathleen M.; Ksiazek, Thomas; Rollin, Pierre E.; Comer, James A.; Lee, William W.; Rota, Paul A.; Bankamp, Bettina; Bellini, William J.; Zaki, Sherif R.

In: Emerging Infectious Diseases, Vol. 10, No. 2, 02.2004, p. 320-326.

Research output: Contribution to journalArticle

Goldsmith, CS, Tatti, KM, Ksiazek, T, Rollin, PE, Comer, JA, Lee, WW, Rota, PA, Bankamp, B, Bellini, WJ & Zaki, SR 2004, 'Ultrastructural Characterization of SARS Coronavirus', Emerging Infectious Diseases, vol. 10, no. 2, pp. 320-326.
Goldsmith CS, Tatti KM, Ksiazek T, Rollin PE, Comer JA, Lee WW et al. Ultrastructural Characterization of SARS Coronavirus. Emerging Infectious Diseases. 2004 Feb;10(2):320-326.
Goldsmith, Cynthia S. ; Tatti, Kathleen M. ; Ksiazek, Thomas ; Rollin, Pierre E. ; Comer, James A. ; Lee, William W. ; Rota, Paul A. ; Bankamp, Bettina ; Bellini, William J. ; Zaki, Sherif R. / Ultrastructural Characterization of SARS Coronavirus. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 2. pp. 320-326.
@article{49e10d3af6b744ae8a405a474512cc46,
title = "Ultrastructural Characterization of SARS Coronavirus",
abstract = "Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.",
author = "Goldsmith, {Cynthia S.} and Tatti, {Kathleen M.} and Thomas Ksiazek and Rollin, {Pierre E.} and Comer, {James A.} and Lee, {William W.} and Rota, {Paul A.} and Bettina Bankamp and Bellini, {William J.} and Zaki, {Sherif R.}",
year = "2004",
month = "2",
language = "English (US)",
volume = "10",
pages = "320--326",
journal = "Emerging Infectious Diseases",
issn = "1080-6040",
publisher = "Centers for Disease Control and Prevention (CDC)",
number = "2",

}

TY - JOUR

T1 - Ultrastructural Characterization of SARS Coronavirus

AU - Goldsmith, Cynthia S.

AU - Tatti, Kathleen M.

AU - Ksiazek, Thomas

AU - Rollin, Pierre E.

AU - Comer, James A.

AU - Lee, William W.

AU - Rota, Paul A.

AU - Bankamp, Bettina

AU - Bellini, William J.

AU - Zaki, Sherif R.

PY - 2004/2

Y1 - 2004/2

N2 - Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.

AB - Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.

UR - http://www.scopus.com/inward/record.url?scp=10744221644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744221644&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 320

EP - 326

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

SN - 1080-6040

IS - 2

ER -