Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica

Walter R A van Heumen, Gregg T. Nagle, Alexander Kurosky

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The atrial gland is an exocrine organ that secretes into the oviduct of Aplysia californica and expresses three homologous genes belonging to the egglaying hormone gene family. Although post-translational processing of the egg-laying hormone precursor in the neuroendocrine bag cells has been examined in detail, relatively little is known about the post-translational processing of egg-laying hormone-related gene products in the atrial gland. A combination of morphologic techniques that included light-microscopic histology and immunocytochemistry, transmission electron microscopy, and immuno-electron microscopy were used to localize egg-laying hormone-related peptides in the atrial gland and to evaluate the characteristic morphology of their secretory cells. Results of these studies showed that there were at least three major types of secretory cells in the atrial gland (types 1-3). Significantly, of these three cell types, only type 1 was immunoreactive to antisera against egg-laying hormone-related precursor peptides. The immunoreactivity studies established that all three egg-laying hormone-related precursor genes are expressed in type-1 cells and indicated that the processing of these precursors also occurs within the secretory granules of this cell type. Evidence was also obtained that proteolytic processing of the egg-laying hormone-related precursors differed significantly from that observed in the bag cells. In contrast to the bag cells, the NH2-terminal and COOH-terminal products of the egg-laying hormone-related precursors of the atrial gland were not sorted into different types of vesicles.

    Original languageEnglish (US)
    Pages (from-to)13-24
    Number of pages12
    JournalCell & Tissue Research
    Volume279
    Issue number1
    DOIs
    StatePublished - Jan 1995

    Fingerprint

    Ovum
    Hormones
    Peptides
    Genes
    Processing
    Exocrine Glands
    Aplysia
    Neuroendocrine Cells
    Oviducts
    Immunoelectron Microscopy
    Peptide Hormones
    Secretory Vesicles
    Histology
    Mollusca egg-laying hormone
    Transmission Electron Microscopy
    Immune Sera
    Electron microscopy
    Immunohistochemistry
    Light
    Transmission electron microscopy

    Keywords

    • Aplysia californica (Mollusca)
    • Atrial gland
    • Egg-laying hormone-related peptides
    • Immunocytochemical localization
    • Prohormone processing

    ASJC Scopus subject areas

    • Anatomy
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica. / van Heumen, Walter R A; Nagle, Gregg T.; Kurosky, Alexander.

    In: Cell & Tissue Research, Vol. 279, No. 1, 01.1995, p. 13-24.

    Research output: Contribution to journalArticle

    van Heumen, Walter R A ; Nagle, Gregg T. ; Kurosky, Alexander. / Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica. In: Cell & Tissue Research. 1995 ; Vol. 279, No. 1. pp. 13-24.
    @article{546ca9fa807440a5b5c6f8de6856d6f7,
    title = "Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica",
    abstract = "The atrial gland is an exocrine organ that secretes into the oviduct of Aplysia californica and expresses three homologous genes belonging to the egglaying hormone gene family. Although post-translational processing of the egg-laying hormone precursor in the neuroendocrine bag cells has been examined in detail, relatively little is known about the post-translational processing of egg-laying hormone-related gene products in the atrial gland. A combination of morphologic techniques that included light-microscopic histology and immunocytochemistry, transmission electron microscopy, and immuno-electron microscopy were used to localize egg-laying hormone-related peptides in the atrial gland and to evaluate the characteristic morphology of their secretory cells. Results of these studies showed that there were at least three major types of secretory cells in the atrial gland (types 1-3). Significantly, of these three cell types, only type 1 was immunoreactive to antisera against egg-laying hormone-related precursor peptides. The immunoreactivity studies established that all three egg-laying hormone-related precursor genes are expressed in type-1 cells and indicated that the processing of these precursors also occurs within the secretory granules of this cell type. Evidence was also obtained that proteolytic processing of the egg-laying hormone-related precursors differed significantly from that observed in the bag cells. In contrast to the bag cells, the NH2-terminal and COOH-terminal products of the egg-laying hormone-related precursors of the atrial gland were not sorted into different types of vesicles.",
    keywords = "Aplysia californica (Mollusca), Atrial gland, Egg-laying hormone-related peptides, Immunocytochemical localization, Prohormone processing",
    author = "{van Heumen}, {Walter R A} and Nagle, {Gregg T.} and Alexander Kurosky",
    year = "1995",
    month = "1",
    doi = "10.1007/BF00300687",
    language = "English (US)",
    volume = "279",
    pages = "13--24",
    journal = "Cell and Tissue Research",
    issn = "0302-766X",
    publisher = "Springer Verlag",
    number = "1",

    }

    TY - JOUR

    T1 - Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica

    AU - van Heumen, Walter R A

    AU - Nagle, Gregg T.

    AU - Kurosky, Alexander

    PY - 1995/1

    Y1 - 1995/1

    N2 - The atrial gland is an exocrine organ that secretes into the oviduct of Aplysia californica and expresses three homologous genes belonging to the egglaying hormone gene family. Although post-translational processing of the egg-laying hormone precursor in the neuroendocrine bag cells has been examined in detail, relatively little is known about the post-translational processing of egg-laying hormone-related gene products in the atrial gland. A combination of morphologic techniques that included light-microscopic histology and immunocytochemistry, transmission electron microscopy, and immuno-electron microscopy were used to localize egg-laying hormone-related peptides in the atrial gland and to evaluate the characteristic morphology of their secretory cells. Results of these studies showed that there were at least three major types of secretory cells in the atrial gland (types 1-3). Significantly, of these three cell types, only type 1 was immunoreactive to antisera against egg-laying hormone-related precursor peptides. The immunoreactivity studies established that all three egg-laying hormone-related precursor genes are expressed in type-1 cells and indicated that the processing of these precursors also occurs within the secretory granules of this cell type. Evidence was also obtained that proteolytic processing of the egg-laying hormone-related precursors differed significantly from that observed in the bag cells. In contrast to the bag cells, the NH2-terminal and COOH-terminal products of the egg-laying hormone-related precursors of the atrial gland were not sorted into different types of vesicles.

    AB - The atrial gland is an exocrine organ that secretes into the oviduct of Aplysia californica and expresses three homologous genes belonging to the egglaying hormone gene family. Although post-translational processing of the egg-laying hormone precursor in the neuroendocrine bag cells has been examined in detail, relatively little is known about the post-translational processing of egg-laying hormone-related gene products in the atrial gland. A combination of morphologic techniques that included light-microscopic histology and immunocytochemistry, transmission electron microscopy, and immuno-electron microscopy were used to localize egg-laying hormone-related peptides in the atrial gland and to evaluate the characteristic morphology of their secretory cells. Results of these studies showed that there were at least three major types of secretory cells in the atrial gland (types 1-3). Significantly, of these three cell types, only type 1 was immunoreactive to antisera against egg-laying hormone-related precursor peptides. The immunoreactivity studies established that all three egg-laying hormone-related precursor genes are expressed in type-1 cells and indicated that the processing of these precursors also occurs within the secretory granules of this cell type. Evidence was also obtained that proteolytic processing of the egg-laying hormone-related precursors differed significantly from that observed in the bag cells. In contrast to the bag cells, the NH2-terminal and COOH-terminal products of the egg-laying hormone-related precursors of the atrial gland were not sorted into different types of vesicles.

    KW - Aplysia californica (Mollusca)

    KW - Atrial gland

    KW - Egg-laying hormone-related peptides

    KW - Immunocytochemical localization

    KW - Prohormone processing

    UR - http://www.scopus.com/inward/record.url?scp=0028847924&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028847924&partnerID=8YFLogxK

    U2 - 10.1007/BF00300687

    DO - 10.1007/BF00300687

    M3 - Article

    VL - 279

    SP - 13

    EP - 24

    JO - Cell and Tissue Research

    JF - Cell and Tissue Research

    SN - 0302-766X

    IS - 1

    ER -