Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.

Vsevolod Popov, A. E. Daiter, N. K. Tokarevich, N. A. Kartseva

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.b.) (strain Apodemus flavicollis-Luga, phase I) or C.b. antigen (formalin-inactivated C.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). Two main types of viable C.b. cells--small dense (DC) and bacteria-like (BC)--were found in omentum, spleen, and peritoneal exudate cells beginning from the 1st day p.i. but maximal amount they reached in spleen at day 14. Some parasitophorous vacuoles contained damaged BC or lysing C.b. cells. C.b. corpuscular antigen was found in phagosomes in ultrathin sections of spleen macrophages until 28 days p.i. By 7 days p.i. in omentum 3 types of parasitophorous phagosomes were described which were supposed to be the sites of the antigen utilization. It is assumed that most BC are destroyed in omentum macrophages during the 1st week after i.p. injection of the antigen, and DC can persist in spleen at least for one month.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalActa Virologica
Volume36
Issue number1
StatePublished - Jan 1992
Externally publishedYes

Fingerprint

Coxiella burnetii
Omentum
Spleen
Antigens
Phagosomes
Infection
Bacteria
Macrophages
Murinae
Peritoneal Macrophages
Exudates and Transudates
Vacuoles
Formaldehyde
Suspensions
Electron Microscopy
Kidney
Injections
Liver

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Popov, V., Daiter, A. E., Tokarevich, N. K., & Kartseva, N. A. (1992). Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice. Acta Virologica, 36(1), 32-38.

Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice. / Popov, Vsevolod; Daiter, A. E.; Tokarevich, N. K.; Kartseva, N. A.

In: Acta Virologica, Vol. 36, No. 1, 01.1992, p. 32-38.

Research output: Contribution to journalArticle

Popov, V, Daiter, AE, Tokarevich, NK & Kartseva, NA 1992, 'Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.', Acta Virologica, vol. 36, no. 1, pp. 32-38.
Popov, Vsevolod ; Daiter, A. E. ; Tokarevich, N. K. ; Kartseva, N. A. / Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice. In: Acta Virologica. 1992 ; Vol. 36, No. 1. pp. 32-38.
@article{2af27a4345f541f5861f72037b834d45,
title = "Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.",
abstract = "C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.b.) (strain Apodemus flavicollis-Luga, phase I) or C.b. antigen (formalin-inactivated C.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). Two main types of viable C.b. cells--small dense (DC) and bacteria-like (BC)--were found in omentum, spleen, and peritoneal exudate cells beginning from the 1st day p.i. but maximal amount they reached in spleen at day 14. Some parasitophorous vacuoles contained damaged BC or lysing C.b. cells. C.b. corpuscular antigen was found in phagosomes in ultrathin sections of spleen macrophages until 28 days p.i. By 7 days p.i. in omentum 3 types of parasitophorous phagosomes were described which were supposed to be the sites of the antigen utilization. It is assumed that most BC are destroyed in omentum macrophages during the 1st week after i.p. injection of the antigen, and DC can persist in spleen at least for one month.",
author = "Vsevolod Popov and Daiter, {A. E.} and Tokarevich, {N. K.} and Kartseva, {N. A.}",
year = "1992",
month = "1",
language = "English (US)",
volume = "36",
pages = "32--38",
journal = "Acta Virologica",
issn = "0001-723X",
publisher = "AEP - Academic Electronic Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.

AU - Popov, Vsevolod

AU - Daiter, A. E.

AU - Tokarevich, N. K.

AU - Kartseva, N. A.

PY - 1992/1

Y1 - 1992/1

N2 - C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.b.) (strain Apodemus flavicollis-Luga, phase I) or C.b. antigen (formalin-inactivated C.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). Two main types of viable C.b. cells--small dense (DC) and bacteria-like (BC)--were found in omentum, spleen, and peritoneal exudate cells beginning from the 1st day p.i. but maximal amount they reached in spleen at day 14. Some parasitophorous vacuoles contained damaged BC or lysing C.b. cells. C.b. corpuscular antigen was found in phagosomes in ultrathin sections of spleen macrophages until 28 days p.i. By 7 days p.i. in omentum 3 types of parasitophorous phagosomes were described which were supposed to be the sites of the antigen utilization. It is assumed that most BC are destroyed in omentum macrophages during the 1st week after i.p. injection of the antigen, and DC can persist in spleen at least for one month.

AB - C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.b.) (strain Apodemus flavicollis-Luga, phase I) or C.b. antigen (formalin-inactivated C.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). Two main types of viable C.b. cells--small dense (DC) and bacteria-like (BC)--were found in omentum, spleen, and peritoneal exudate cells beginning from the 1st day p.i. but maximal amount they reached in spleen at day 14. Some parasitophorous vacuoles contained damaged BC or lysing C.b. cells. C.b. corpuscular antigen was found in phagosomes in ultrathin sections of spleen macrophages until 28 days p.i. By 7 days p.i. in omentum 3 types of parasitophorous phagosomes were described which were supposed to be the sites of the antigen utilization. It is assumed that most BC are destroyed in omentum macrophages during the 1st week after i.p. injection of the antigen, and DC can persist in spleen at least for one month.

UR - http://www.scopus.com/inward/record.url?scp=0026478480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026478480&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 32

EP - 38

JO - Acta Virologica

JF - Acta Virologica

SN - 0001-723X

IS - 1

ER -