Umatilla virus genome sequencing and phylogenetic analysis

Identification of Stretch Lagoon orbivirus as a new member of the Umatilla virus species

Manjunatha N. Belaganahalli, Sushila Maan, Narender S. Maan, Robert Tesh, Houssam Attoui, Peter P C Mertens

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The genus Orbivirus, family Reoviridae, includes 22 species of viruses with genomes composed of ten segments of linear dsRNA that are transmitted between their vertebrate hosts by insects or ticks, or with no identified vectors. Full-genome sequence data are available for representative isolates of the insect borne mammalian orbiviruses (including bluetongue virus), as well as a tick borne avian orbivirus (Great Island virus). However, no sequence data are as yet available for the mosquito borne avian orbiviruses. We report full-length, whole-genome sequence data for Umatilla virus (UMAV), a mosquito borne avian orbivirus from the USA, which belongs to the species Umatilla virus. Comparisons of conserved genome segments 1, 2 and 8 (Seg-1, Seg-2 and Seg-8) - encoding the polymerase-VP1, sub-core 'T2' protein and core-surface 'T13' protein, respectively, show that UMAV groups with the mosquito transmitted mammalian orbiviruses. The highest levels of sequence identity were detected between UMAV and Stretch Lagoon orbivirus (SLOV) from Australia, showing that they belong to the same virus species (with nt/aa identity of 76.04%/88.07% and 77.96%/95.36% in the polymerase and T2 genes and protein, respectively). The data presented here has assisted in identifying the SLOV as a member of the Umatilla serogroup. This sequence data reported here will also facilitate identification of new isolates, and epidemiological studies of viruses belonging to the species Umatilla virus.

Original languageEnglish (US)
Article numbere23605
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - Aug 29 2011

Fingerprint

Umatilla virus
Orbivirus
Viruses
Genes
Genome
genome
phylogeny
Culicidae
Great Island virus
Ticks
viruses
ticks
Insects
Reoviridae
Bluetongue virus
insects
double-stranded RNA
surface proteins
Proteins
epidemiological studies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Umatilla virus genome sequencing and phylogenetic analysis : Identification of Stretch Lagoon orbivirus as a new member of the Umatilla virus species. / Belaganahalli, Manjunatha N.; Maan, Sushila; Maan, Narender S.; Tesh, Robert; Attoui, Houssam; Mertens, Peter P C.

In: PLoS One, Vol. 6, No. 8, e23605, 29.08.2011.

Research output: Contribution to journalArticle

Belaganahalli, Manjunatha N. ; Maan, Sushila ; Maan, Narender S. ; Tesh, Robert ; Attoui, Houssam ; Mertens, Peter P C. / Umatilla virus genome sequencing and phylogenetic analysis : Identification of Stretch Lagoon orbivirus as a new member of the Umatilla virus species. In: PLoS One. 2011 ; Vol. 6, No. 8.
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