Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies

Xia Cao; Junki Maruyama; Heyue Zhou; Yanwen Fu; Lisa Kerwin; Colin Powers; Rachel A. Sattler; John T. Manning; Alok Singh; Reyna Lim; Laura D. Healy; Sachi Johnson; Elizabeth Paz Cabral; Donghui Li; Lucy Lu; Arthur Ledesma; Daniel Lee; Susan Richards; Laura Rivero-Nava; Yan Li; Weiqun Shen; Karen Stegman; Benjamin Blair; Shinji Urata; Magumi Kishimoto-Urata; Jamie Ko; Na Du; Kyndal Morais; Kate Lawrence; Ianne Rivera; Chin I. Pai; Damien Bresson; Mark Brunswick; Yanliang Zhang; Henry Ji; Slobodan Paessler; Robert D. Allen

doi:10.1038/s41598-022-19780-7

Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies

Xia Cao
, Junki Maruyama
, Heyue Zhou
, Yanwen Fu
, Lisa Kerwin
, Colin Powers
, Rachel A. Sattler
, John T. Manning
, Alok Singh
, Reyna Lim
, Laura D. Healy
, Sachi Johnson
, Elizabeth Paz Cabral
, Donghui Li
, Lucy Lu
, Arthur Ledesma
, Daniel Lee
, Susan Richards
, Laura Rivero-Nava
, Yan Li

Weiqun Shen, Karen Stegman, Benjamin Blair, Shinji Urata, Magumi Kishimoto-Urata, Jamie Ko, Na Du, Kyndal Morais, Kate Lawrence, Ianne Rivera, Chin I. Pai, Damien Bresson, Mark Brunswick, Yanliang Zhang, Henry Ji, Slobodan Paessler, Robert D. Allen

Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Coronavirus disease 2019 (COVID-19) continues to significantly impact the global population, thus countermeasure platforms that enable rapid development of therapeutics against variants of SARS-CoV-2 are essential. We report use of a phage display human antibody library approach to rapidly identify neutralizing antibodies (nAbs) against SARS-CoV-2. We demonstrate the binding and neutralization capability of two nAbs, STI-2020 and STI-5041, against the SARS-CoV-2 WA-1 strain as well as the Alpha and Beta variants. STI-2020 and STI-5041 were protective when administered intravenously or intranasally in the golden (Syrian) hamster model of COVID-19 challenged with the WA-1 strain or Beta variant. The ability to administer nAbs intravenously and intranasally may have important therapeutic implications and Phase 1 healthy subjects clinical trials are ongoing.

Original language	English (US)
Article number	15517
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-19780-7
State	Published - Dec 2022

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Cao, X., Maruyama, J., Zhou, H., Fu, Y., Kerwin, L., Powers, C., Sattler, R. A., Manning, J. T., Singh, A., Lim, R., Healy, L. D., Johnson, S., Paz Cabral, E., Li, D., Lu, L., Ledesma, A., Lee, D., Richards, S., Rivero-Nava, L., ... Allen, R. D. (2022). Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies. Scientific reports, 12(1), Article 15517. https://doi.org/10.1038/s41598-022-19780-7

Cao, X, Maruyama, J, Zhou, H, Fu, Y, Kerwin, L, Powers, C, Sattler, RA, Manning, JT, Singh, A, Lim, R, Healy, LD, Johnson, S, Paz Cabral, E, Li, D, Lu, L, Ledesma, A, Lee, D, Richards, S, Rivero-Nava, L, Li, Y, Shen, W, Stegman, K, Blair, B, Urata, S, Kishimoto-Urata, M, Ko, J, Du, N, Morais, K, Lawrence, K, Rivera, I, Pai, CI, Bresson, D, Brunswick, M, Zhang, Y, Ji, H, Paessler, S & Allen, RD 2022, 'Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies', Scientific reports, vol. 12, no. 1, 15517. https://doi.org/10.1038/s41598-022-19780-7

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title = "Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies",

abstract = "Coronavirus disease 2019 (COVID-19) continues to significantly impact the global population, thus countermeasure platforms that enable rapid development of therapeutics against variants of SARS-CoV-2 are essential. We report use of a phage display human antibody library approach to rapidly identify neutralizing antibodies (nAbs) against SARS-CoV-2. We demonstrate the binding and neutralization capability of two nAbs, STI-2020 and STI-5041, against the SARS-CoV-2 WA-1 strain as well as the Alpha and Beta variants. STI-2020 and STI-5041 were protective when administered intravenously or intranasally in the golden (Syrian) hamster model of COVID-19 challenged with the WA-1 strain or Beta variant. The ability to administer nAbs intravenously and intranasally may have important therapeutic implications and Phase 1 healthy subjects clinical trials are ongoing.",

author = "Xia Cao and Junki Maruyama and Heyue Zhou and Yanwen Fu and Lisa Kerwin and Colin Powers and Sattler, \{Rachel A.\} and Manning, \{John T.\} and Alok Singh and Reyna Lim and Healy, \{Laura D.\} and Sachi Johnson and \{Paz Cabral\}, Elizabeth and Donghui Li and Lucy Lu and Arthur Ledesma and Daniel Lee and Susan Richards and Laura Rivero-Nava and Yan Li and Weiqun Shen and Karen Stegman and Benjamin Blair and Shinji Urata and Magumi Kishimoto-Urata and Jamie Ko and Na Du and Kyndal Morais and Kate Lawrence and Ianne Rivera and Pai, \{Chin I.\} and Damien Bresson and Mark Brunswick and Yanliang Zhang and Henry Ji and Slobodan Paessler and Allen, \{Robert D.\}",

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AU - Zhou, Heyue

AU - Fu, Yanwen

AU - Kerwin, Lisa

AU - Powers, Colin

AU - Sattler, Rachel A.

AU - Manning, John T.

AU - Singh, Alok

AU - Lim, Reyna

AU - Healy, Laura D.

AU - Johnson, Sachi

AU - Paz Cabral, Elizabeth

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AU - Lee, Daniel

AU - Richards, Susan

AU - Rivero-Nava, Laura

AU - Li, Yan

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AU - Stegman, Karen

AU - Blair, Benjamin

AU - Urata, Shinji

AU - Kishimoto-Urata, Magumi

AU - Ko, Jamie

AU - Du, Na

AU - Morais, Kyndal

AU - Lawrence, Kate

AU - Rivera, Ianne

AU - Pai, Chin I.

AU - Bresson, Damien

AU - Brunswick, Mark

AU - Zhang, Yanliang

AU - Ji, Henry

AU - Paessler, Slobodan

AU - Allen, Robert D.

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Unbiased approach to identify and assess efficacy of human SARS-CoV-2 neutralizing antibodies

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