Uncovering the mechanism of trapping and cell orientation during Neisseria gonorrhoeae twitching motility

Vasily Zaburdaev, Nicolas Biais, Michael Schmiedeberg, Jens Eriksson, Ann Beth Jonsson, Michael Sheetz, David A. Weitz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Neisseria gonorrheae bacteria are the causative agent of the second most common sexually transmitted infection in the world. The bacteria move on a surface by means of twitching motility. Their movement is mediated by multiple long and flexible filaments, called type IV pili, that extend from the cell body, attach to the surface, and retract, thus generating a pulling force. Moving cells also use pili to aggregate and form microcolonies. However, the mechanism by which the pili surrounding the cell body work together to propel bacteria remains unclear. Understanding this process will help describe the motility of N. gonorrheae bacteria, and thus the dissemination of the disease which they cause. In this article we track individual twitching cells and observe that their trajectories consist of alternating moving and pausing intervals, while the cell body is preferably oriented with its wide side toward the direction of motion. Based on these data, we propose a model for the collective pili operation of N. gonorrheae bacteria that explains the experimentally observed behavior. Individual pili function independently but can lead to coordinated motion or pausing via the force balance. The geometry of the cell defines its orientation during motion. We show that by changing pili substrate interactions, the motility pattern can be altered in a predictable way. Although the model proposed is tangibly simple, it still has sufficient robustness to incorporate further advanced pili features and various cell geometries to describe other bacteria that employ pili to move on surfaces.

Original languageEnglish (US)
Pages (from-to)1523-1531
Number of pages9
JournalBiophysical Journal
Volume107
Issue number7
DOIs
StatePublished - Oct 7 2014
Externally publishedYes

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Neisseria gonorrhoeae
Bacteria
Sexually Transmitted Diseases
Cell Body

ASJC Scopus subject areas

  • Biophysics

Cite this

Zaburdaev, V., Biais, N., Schmiedeberg, M., Eriksson, J., Jonsson, A. B., Sheetz, M., & Weitz, D. A. (2014). Uncovering the mechanism of trapping and cell orientation during Neisseria gonorrhoeae twitching motility. Biophysical Journal, 107(7), 1523-1531. https://doi.org/10.1016/j.bpj.2014.07.061

Uncovering the mechanism of trapping and cell orientation during Neisseria gonorrhoeae twitching motility. / Zaburdaev, Vasily; Biais, Nicolas; Schmiedeberg, Michael; Eriksson, Jens; Jonsson, Ann Beth; Sheetz, Michael; Weitz, David A.

In: Biophysical Journal, Vol. 107, No. 7, 07.10.2014, p. 1523-1531.

Research output: Contribution to journalArticle

Zaburdaev, V, Biais, N, Schmiedeberg, M, Eriksson, J, Jonsson, AB, Sheetz, M & Weitz, DA 2014, 'Uncovering the mechanism of trapping and cell orientation during Neisseria gonorrhoeae twitching motility', Biophysical Journal, vol. 107, no. 7, pp. 1523-1531. https://doi.org/10.1016/j.bpj.2014.07.061
Zaburdaev, Vasily ; Biais, Nicolas ; Schmiedeberg, Michael ; Eriksson, Jens ; Jonsson, Ann Beth ; Sheetz, Michael ; Weitz, David A. / Uncovering the mechanism of trapping and cell orientation during Neisseria gonorrhoeae twitching motility. In: Biophysical Journal. 2014 ; Vol. 107, No. 7. pp. 1523-1531.
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