Understanding local Mediterranean diets

A multidisciplinary pharmacological and ethnobotanical approach

Sebastian Schaffer, Michael Heinrich, Marco Leonti, Sabine Nebel, Wieland Peschel, Andrea Pieroni, Felicity Smith, Diego Rivera, Concepción Obón, Cristina Inocencio, Alonso Verde, José Fajardo, Rafael Llorach, Walter E. Müller, Gunter P. Eckert, Stephanie Schmitt-Schillig, Smaragda Antonopoulou, Zacharias Kypriotakis, Yannis Manios, Tzortzis Nomikos & 15 others Andriana Kaliora, Labros Sidossis, Claudio Galli, Francesco Visioli, Simona Grande, Paola Bogani, Antoine de Saizieu, Beat Flühmann, Daniel D'Orazio, Ann Fowler, Aleksander Koj, Joanna Bereta, Józef Dulak, Amalia Guzdek, Maria Kapiszewska

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Epidemiological data indicate a beneficial effect of Mediterranean diets on human health, especially on the prevalence of cardiovascular disease. These observations are supported by recent intervention studies. However, very little is known about the current role of local Mediterranean food products, which are consumed on a less regular basis and their contribution to a healthy diet. The European consortium "Local Food-Nutraceuticals" collected 127 locally consumed wild or semi-wild plants in three Mediterranean countries, i.e. Greece, Italy, and Spain, in order to assess their ethnobotanical features as well as their biological activities. The project also includes a second line of research, the study of local conceptions about these food resources. All pharmacological assays were conducted with ethanolic extracts prepared from the dried plant material. The biological activities of the extracts were assessed with the following 12 different assays covering a broad range of mechanisms considered crucial in the pathology of chronic, aging-related diseases. Four antioxidant tests: DPPH scavenging, prevention of oxyhaemoglobin bleaching, prevention of lipid peroxidation (malondialdehyde formation), and protection from DNA damage (Comet assay); three enzyme inhibition tests: inhibition of xanthine oxidase, inhibition of myeloperoxidase-catalysed guaiacol oxidation as well as the inhibition of acetylcholine esterase; one test investigating the inhibition of cytokine-induced cell activation (including the extracts' potential cytotoxicity); one assay measuring the anti-proliferation potential; one test assessing the anti-diabetic activity (PPARγ) as well as one assay investigating the extracts' effect on mood disorder-related biochemical parameters (hSERT). Furthermore, the polyphenol content of all extracts was determined using the Folin-Ciocalteaus method. The assays revealed diverse biological effects for the tested extracts ranging from no activity to almost complete inhibition/activation. Moreover, the experimental matrix led to the identification of a sub-set of extracts, i.e. Berberis vulgaris, Reichardia picroides, Scandix australis, Satureja montana, Thymus piperella, Lythrum salicaria and Vitis vinifera, showing high activity in a broad range of assays. In summary, the in vitro observed modulations and effects exerted by extracts derived from local food plants suggest that these plants may contribute to the observed better aging of rural Mediterranean populations.

Original languageEnglish (US)
Pages (from-to)353-366
Number of pages14
JournalPharmacological Research
Volume52
Issue number4
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Mediterranean Diet
Pharmacology
Food
Lythrum
Berberis
Satureja
Guaiacol
Oxyhemoglobins
Edible Plants
Peroxisome Proliferator-Activated Receptors
Comet Assay
Xanthine Oxidase
Greece
Vitis
Polyphenols
Rural Population
Esterases
Dietary Supplements
Malondialdehyde
Mood Disorders

Keywords

  • Antioxidants
  • Ethnopharmacology
  • Food plants
  • Local food
  • Mediterranean diet(s)
  • Nutraceuticals
  • Polyphenols

ASJC Scopus subject areas

  • Pharmacology

Cite this

Schaffer, S., Heinrich, M., Leonti, M., Nebel, S., Peschel, W., Pieroni, A., ... Kapiszewska, M. (2005). Understanding local Mediterranean diets: A multidisciplinary pharmacological and ethnobotanical approach. Pharmacological Research, 52(4), 353-366. https://doi.org/10.1016/j.phrs.2005.06.005

Understanding local Mediterranean diets : A multidisciplinary pharmacological and ethnobotanical approach. / Schaffer, Sebastian; Heinrich, Michael; Leonti, Marco; Nebel, Sabine; Peschel, Wieland; Pieroni, Andrea; Smith, Felicity; Rivera, Diego; Obón, Concepción; Inocencio, Cristina; Verde, Alonso; Fajardo, José; Llorach, Rafael; Müller, Walter E.; Eckert, Gunter P.; Schmitt-Schillig, Stephanie; Antonopoulou, Smaragda; Kypriotakis, Zacharias; Manios, Yannis; Nomikos, Tzortzis; Kaliora, Andriana; Sidossis, Labros; Galli, Claudio; Visioli, Francesco; Grande, Simona; Bogani, Paola; de Saizieu, Antoine; Flühmann, Beat; D'Orazio, Daniel; Fowler, Ann; Koj, Aleksander; Bereta, Joanna; Dulak, Józef; Guzdek, Amalia; Kapiszewska, Maria.

In: Pharmacological Research, Vol. 52, No. 4, 10.2005, p. 353-366.

Research output: Contribution to journalArticle

Schaffer, S, Heinrich, M, Leonti, M, Nebel, S, Peschel, W, Pieroni, A, Smith, F, Rivera, D, Obón, C, Inocencio, C, Verde, A, Fajardo, J, Llorach, R, Müller, WE, Eckert, GP, Schmitt-Schillig, S, Antonopoulou, S, Kypriotakis, Z, Manios, Y, Nomikos, T, Kaliora, A, Sidossis, L, Galli, C, Visioli, F, Grande, S, Bogani, P, de Saizieu, A, Flühmann, B, D'Orazio, D, Fowler, A, Koj, A, Bereta, J, Dulak, J, Guzdek, A & Kapiszewska, M 2005, 'Understanding local Mediterranean diets: A multidisciplinary pharmacological and ethnobotanical approach', Pharmacological Research, vol. 52, no. 4, pp. 353-366. https://doi.org/10.1016/j.phrs.2005.06.005
Schaffer, Sebastian ; Heinrich, Michael ; Leonti, Marco ; Nebel, Sabine ; Peschel, Wieland ; Pieroni, Andrea ; Smith, Felicity ; Rivera, Diego ; Obón, Concepción ; Inocencio, Cristina ; Verde, Alonso ; Fajardo, José ; Llorach, Rafael ; Müller, Walter E. ; Eckert, Gunter P. ; Schmitt-Schillig, Stephanie ; Antonopoulou, Smaragda ; Kypriotakis, Zacharias ; Manios, Yannis ; Nomikos, Tzortzis ; Kaliora, Andriana ; Sidossis, Labros ; Galli, Claudio ; Visioli, Francesco ; Grande, Simona ; Bogani, Paola ; de Saizieu, Antoine ; Flühmann, Beat ; D'Orazio, Daniel ; Fowler, Ann ; Koj, Aleksander ; Bereta, Joanna ; Dulak, Józef ; Guzdek, Amalia ; Kapiszewska, Maria. / Understanding local Mediterranean diets : A multidisciplinary pharmacological and ethnobotanical approach. In: Pharmacological Research. 2005 ; Vol. 52, No. 4. pp. 353-366.
@article{7f440ab571644f9f8979d7f9153f95a3,
title = "Understanding local Mediterranean diets: A multidisciplinary pharmacological and ethnobotanical approach",
abstract = "Epidemiological data indicate a beneficial effect of Mediterranean diets on human health, especially on the prevalence of cardiovascular disease. These observations are supported by recent intervention studies. However, very little is known about the current role of local Mediterranean food products, which are consumed on a less regular basis and their contribution to a healthy diet. The European consortium {"}Local Food-Nutraceuticals{"} collected 127 locally consumed wild or semi-wild plants in three Mediterranean countries, i.e. Greece, Italy, and Spain, in order to assess their ethnobotanical features as well as their biological activities. The project also includes a second line of research, the study of local conceptions about these food resources. All pharmacological assays were conducted with ethanolic extracts prepared from the dried plant material. The biological activities of the extracts were assessed with the following 12 different assays covering a broad range of mechanisms considered crucial in the pathology of chronic, aging-related diseases. Four antioxidant tests: DPPH scavenging, prevention of oxyhaemoglobin bleaching, prevention of lipid peroxidation (malondialdehyde formation), and protection from DNA damage (Comet assay); three enzyme inhibition tests: inhibition of xanthine oxidase, inhibition of myeloperoxidase-catalysed guaiacol oxidation as well as the inhibition of acetylcholine esterase; one test investigating the inhibition of cytokine-induced cell activation (including the extracts' potential cytotoxicity); one assay measuring the anti-proliferation potential; one test assessing the anti-diabetic activity (PPARγ) as well as one assay investigating the extracts' effect on mood disorder-related biochemical parameters (hSERT). Furthermore, the polyphenol content of all extracts was determined using the Folin-Ciocalteaus method. The assays revealed diverse biological effects for the tested extracts ranging from no activity to almost complete inhibition/activation. Moreover, the experimental matrix led to the identification of a sub-set of extracts, i.e. Berberis vulgaris, Reichardia picroides, Scandix australis, Satureja montana, Thymus piperella, Lythrum salicaria and Vitis vinifera, showing high activity in a broad range of assays. In summary, the in vitro observed modulations and effects exerted by extracts derived from local food plants suggest that these plants may contribute to the observed better aging of rural Mediterranean populations.",
keywords = "Antioxidants, Ethnopharmacology, Food plants, Local food, Mediterranean diet(s), Nutraceuticals, Polyphenols",
author = "Sebastian Schaffer and Michael Heinrich and Marco Leonti and Sabine Nebel and Wieland Peschel and Andrea Pieroni and Felicity Smith and Diego Rivera and Concepci{\'o}n Ob{\'o}n and Cristina Inocencio and Alonso Verde and Jos{\'e} Fajardo and Rafael Llorach and M{\"u}ller, {Walter E.} and Eckert, {Gunter P.} and Stephanie Schmitt-Schillig and Smaragda Antonopoulou and Zacharias Kypriotakis and Yannis Manios and Tzortzis Nomikos and Andriana Kaliora and Labros Sidossis and Claudio Galli and Francesco Visioli and Simona Grande and Paola Bogani and {de Saizieu}, Antoine and Beat Fl{\"u}hmann and Daniel D'Orazio and Ann Fowler and Aleksander Koj and Joanna Bereta and J{\'o}zef Dulak and Amalia Guzdek and Maria Kapiszewska",
year = "2005",
month = "10",
doi = "10.1016/j.phrs.2005.06.005",
language = "English (US)",
volume = "52",
pages = "353--366",
journal = "Pharmacological Research",
issn = "1043-6618",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Understanding local Mediterranean diets

T2 - A multidisciplinary pharmacological and ethnobotanical approach

AU - Schaffer, Sebastian

AU - Heinrich, Michael

AU - Leonti, Marco

AU - Nebel, Sabine

AU - Peschel, Wieland

AU - Pieroni, Andrea

AU - Smith, Felicity

AU - Rivera, Diego

AU - Obón, Concepción

AU - Inocencio, Cristina

AU - Verde, Alonso

AU - Fajardo, José

AU - Llorach, Rafael

AU - Müller, Walter E.

AU - Eckert, Gunter P.

AU - Schmitt-Schillig, Stephanie

AU - Antonopoulou, Smaragda

AU - Kypriotakis, Zacharias

AU - Manios, Yannis

AU - Nomikos, Tzortzis

AU - Kaliora, Andriana

AU - Sidossis, Labros

AU - Galli, Claudio

AU - Visioli, Francesco

AU - Grande, Simona

AU - Bogani, Paola

AU - de Saizieu, Antoine

AU - Flühmann, Beat

AU - D'Orazio, Daniel

AU - Fowler, Ann

AU - Koj, Aleksander

AU - Bereta, Joanna

AU - Dulak, Józef

AU - Guzdek, Amalia

AU - Kapiszewska, Maria

PY - 2005/10

Y1 - 2005/10

N2 - Epidemiological data indicate a beneficial effect of Mediterranean diets on human health, especially on the prevalence of cardiovascular disease. These observations are supported by recent intervention studies. However, very little is known about the current role of local Mediterranean food products, which are consumed on a less regular basis and their contribution to a healthy diet. The European consortium "Local Food-Nutraceuticals" collected 127 locally consumed wild or semi-wild plants in three Mediterranean countries, i.e. Greece, Italy, and Spain, in order to assess their ethnobotanical features as well as their biological activities. The project also includes a second line of research, the study of local conceptions about these food resources. All pharmacological assays were conducted with ethanolic extracts prepared from the dried plant material. The biological activities of the extracts were assessed with the following 12 different assays covering a broad range of mechanisms considered crucial in the pathology of chronic, aging-related diseases. Four antioxidant tests: DPPH scavenging, prevention of oxyhaemoglobin bleaching, prevention of lipid peroxidation (malondialdehyde formation), and protection from DNA damage (Comet assay); three enzyme inhibition tests: inhibition of xanthine oxidase, inhibition of myeloperoxidase-catalysed guaiacol oxidation as well as the inhibition of acetylcholine esterase; one test investigating the inhibition of cytokine-induced cell activation (including the extracts' potential cytotoxicity); one assay measuring the anti-proliferation potential; one test assessing the anti-diabetic activity (PPARγ) as well as one assay investigating the extracts' effect on mood disorder-related biochemical parameters (hSERT). Furthermore, the polyphenol content of all extracts was determined using the Folin-Ciocalteaus method. The assays revealed diverse biological effects for the tested extracts ranging from no activity to almost complete inhibition/activation. Moreover, the experimental matrix led to the identification of a sub-set of extracts, i.e. Berberis vulgaris, Reichardia picroides, Scandix australis, Satureja montana, Thymus piperella, Lythrum salicaria and Vitis vinifera, showing high activity in a broad range of assays. In summary, the in vitro observed modulations and effects exerted by extracts derived from local food plants suggest that these plants may contribute to the observed better aging of rural Mediterranean populations.

AB - Epidemiological data indicate a beneficial effect of Mediterranean diets on human health, especially on the prevalence of cardiovascular disease. These observations are supported by recent intervention studies. However, very little is known about the current role of local Mediterranean food products, which are consumed on a less regular basis and their contribution to a healthy diet. The European consortium "Local Food-Nutraceuticals" collected 127 locally consumed wild or semi-wild plants in three Mediterranean countries, i.e. Greece, Italy, and Spain, in order to assess their ethnobotanical features as well as their biological activities. The project also includes a second line of research, the study of local conceptions about these food resources. All pharmacological assays were conducted with ethanolic extracts prepared from the dried plant material. The biological activities of the extracts were assessed with the following 12 different assays covering a broad range of mechanisms considered crucial in the pathology of chronic, aging-related diseases. Four antioxidant tests: DPPH scavenging, prevention of oxyhaemoglobin bleaching, prevention of lipid peroxidation (malondialdehyde formation), and protection from DNA damage (Comet assay); three enzyme inhibition tests: inhibition of xanthine oxidase, inhibition of myeloperoxidase-catalysed guaiacol oxidation as well as the inhibition of acetylcholine esterase; one test investigating the inhibition of cytokine-induced cell activation (including the extracts' potential cytotoxicity); one assay measuring the anti-proliferation potential; one test assessing the anti-diabetic activity (PPARγ) as well as one assay investigating the extracts' effect on mood disorder-related biochemical parameters (hSERT). Furthermore, the polyphenol content of all extracts was determined using the Folin-Ciocalteaus method. The assays revealed diverse biological effects for the tested extracts ranging from no activity to almost complete inhibition/activation. Moreover, the experimental matrix led to the identification of a sub-set of extracts, i.e. Berberis vulgaris, Reichardia picroides, Scandix australis, Satureja montana, Thymus piperella, Lythrum salicaria and Vitis vinifera, showing high activity in a broad range of assays. In summary, the in vitro observed modulations and effects exerted by extracts derived from local food plants suggest that these plants may contribute to the observed better aging of rural Mediterranean populations.

KW - Antioxidants

KW - Ethnopharmacology

KW - Food plants

KW - Local food

KW - Mediterranean diet(s)

KW - Nutraceuticals

KW - Polyphenols

UR - http://www.scopus.com/inward/record.url?scp=23844525962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844525962&partnerID=8YFLogxK

U2 - 10.1016/j.phrs.2005.06.005

DO - 10.1016/j.phrs.2005.06.005

M3 - Article

VL - 52

SP - 353

EP - 366

JO - Pharmacological Research

JF - Pharmacological Research

SN - 1043-6618

IS - 4

ER -