Unstable hemoglobin hemolytic anemia: In vitro incubation studies on erythrocytes with hemoglobin Sabine

Gordon C. Mills, Jack B. Alperin, Freddie L. Hill, Ralph J. Henderson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Additional biochemical studies have been carried out to investigate the effects of the presence of an unstable hemoglobin (Hb Sabine) on metabolism of erythrocytes. In vitro incubation under physiological conditions of these erythrocytes for periods of 5-15 hours has been utilized to evaluate effects of various additives on metabolism of the cells. The addition of adenine to the blood stimulates adenine nucleotide biosynthesis and is effective in maintaining levels of adenine nucleotides. An excessive rate of breakdown of adenine nucleotides to hypoxanthine has been shown previously to be a major factor in the inability of these cells to maintain ATP. The addition of azide during in vitro incubation proved detrimental to the metabolism of these abnormal erythrocytes. This finding indicates that the immature erythrocytes of the blood are relying on oxidative phosphorylation to meet a portion of their energy needs. Neither pyruvate nor sucrose provided significant beneficial effects on the metabolism of the cells. Methylene blue stimulated the formation of 2,3-diphosphoglycerate, but proved harmful to the maintenance of adenine nucleotides. Effects of precipitation of denatured globin from hemoglobin Sabine on reduced glutathione and on the sulfhydryl enzyme, glyceraldehyde-3-phosphate dehydrogenase, were also studied following in vitro incubation. The precipitating beta polypeptide chains apparently do form disulfide bridges with reduced glutathione. However, no disulfide bridge formation with glyceraldehyde-3-phosphate dehydrogenase was noted during a 15-hour in vitro incubation.

Original languageEnglish (US)
Pages (from-to)212-226
Number of pages15
JournalBiochemical Medicine
Volume5
Issue number3
DOIs
StatePublished - 1971

Fingerprint

Adenine Nucleotides
Hemolytic Anemia
Metabolism
Hemoglobins
Erythrocytes
Glyceraldehyde-3-Phosphate Dehydrogenases
Disulfides
Glutathione
Abnormal Erythrocytes
Blood
2,3-Diphosphoglycerate
Hypoxanthine
Globins
Azides
Methylene Blue
Biosynthesis
Oxidative Phosphorylation
Adenine
Pyruvic Acid
Sucrose

ASJC Scopus subject areas

  • Biochemistry

Cite this

Unstable hemoglobin hemolytic anemia : In vitro incubation studies on erythrocytes with hemoglobin Sabine. / Mills, Gordon C.; Alperin, Jack B.; Hill, Freddie L.; Henderson, Ralph J.

In: Biochemical Medicine, Vol. 5, No. 3, 1971, p. 212-226.

Research output: Contribution to journalArticle

Mills, Gordon C. ; Alperin, Jack B. ; Hill, Freddie L. ; Henderson, Ralph J. / Unstable hemoglobin hemolytic anemia : In vitro incubation studies on erythrocytes with hemoglobin Sabine. In: Biochemical Medicine. 1971 ; Vol. 5, No. 3. pp. 212-226.
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