Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers

Claudia Corbo, Roberto Molinaro, Francesca Taraballi, Naama E. Toledano Furman, Kelly A. Hartman, Michael Sherman, Enrica De Rosa, Dickson K. Kirui, Francesco Salvatore, Ennio Tasciotti

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Understanding interactions occurring at the interface between nanoparticles and biological components is an urgent challenge in nanomedicine due to their effect on the biological fate of nanoparticles. After the systemic injection of nanoparticles, a protein corona constructed by blood components surrounds the carrier's surface and modulates its pharmacokinetics and biodistribution. Biomimicry-based approaches in nanotechnology attempt to imitate what happens in nature in order to transfer specific natural functionalities to synthetic nanoparticles. Several biomimetic formulations have been developed, showing superior in vivo features as a result of their cell-like identity. We have recently designed biomimetic liposomes, called leukosomes, which recapitulate the ability of leukocytes to target inflamed endothelium and escape clearance by the immune system. To gain insight into the properties of leukosomes, we decided to investigate their protein corona in vivo. So far, most information about the protein corona has been obtained using in vitro experiments, which have been shown to minimally reproduce in vivo phenomena. Here we directly show a time-dependent quantitative and qualitative analysis of the protein corona adsorbed in vivo on leukosomes and control liposomes. We observed that leukosomes absorb fewer proteins than liposomes, and we identified a group of proteins specifically adsorbed on leukosomes. Moreover, we hypothesize that the presence of macrophage receptors on leukosomes' surface neutralizes their protein corona-meditated uptake by immune cells. This work unveils the protein corona of a biomimetic carrier and is one of the few studies on the corona performed in vivo.

Original languageEnglish (US)
Pages (from-to)3262-3273
Number of pages12
JournalACS Nano
Volume11
Issue number3
DOIs
StatePublished - Mar 28 2017

Fingerprint

leukocytes
coronas
proteins
Proteins
Biomimetics
Liposomes
Nanoparticles
biomimetics
nanoparticles
Medical nanotechnology
Pharmacokinetics
Macrophages
Immune system
endothelium
Nanotechnology
immune systems
macrophages
Protein Corona
qualitative analysis
clearances

Keywords

  • biomimicry
  • in vivo corona
  • leukosome
  • liposome
  • macrophages
  • nanomedicine
  • protein corona

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Corbo, C., Molinaro, R., Taraballi, F., Toledano Furman, N. E., Hartman, K. A., Sherman, M., ... Tasciotti, E. (2017). Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers. ACS Nano, 11(3), 3262-3273. https://doi.org/10.1021/acsnano.7b00376

Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers. / Corbo, Claudia; Molinaro, Roberto; Taraballi, Francesca; Toledano Furman, Naama E.; Hartman, Kelly A.; Sherman, Michael; De Rosa, Enrica; Kirui, Dickson K.; Salvatore, Francesco; Tasciotti, Ennio.

In: ACS Nano, Vol. 11, No. 3, 28.03.2017, p. 3262-3273.

Research output: Contribution to journalArticle

Corbo, C, Molinaro, R, Taraballi, F, Toledano Furman, NE, Hartman, KA, Sherman, M, De Rosa, E, Kirui, DK, Salvatore, F & Tasciotti, E 2017, 'Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers', ACS Nano, vol. 11, no. 3, pp. 3262-3273. https://doi.org/10.1021/acsnano.7b00376
Corbo C, Molinaro R, Taraballi F, Toledano Furman NE, Hartman KA, Sherman M et al. Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers. ACS Nano. 2017 Mar 28;11(3):3262-3273. https://doi.org/10.1021/acsnano.7b00376
Corbo, Claudia ; Molinaro, Roberto ; Taraballi, Francesca ; Toledano Furman, Naama E. ; Hartman, Kelly A. ; Sherman, Michael ; De Rosa, Enrica ; Kirui, Dickson K. ; Salvatore, Francesco ; Tasciotti, Ennio. / Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers. In: ACS Nano. 2017 ; Vol. 11, No. 3. pp. 3262-3273.
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