Urinary excretion of modified purines and nucleosides in immunodeficient children

Gordon C. Mills, Frank C. Schmalstieg, Randall M. Goldblum

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Studies have been carried out using an XAD-4 resin and ion-exchange chromatography for determination of urinary purines and nucleosides in seven children with severe combined immunodeficiency and in six normal children. These studies have included analyses for five methylated purines or nucleosides produced by catabolism of nucleic acids. The following compounds have been quantitatively determined: 1-methyladenosine, 1-methylinosine, 1-methylguanosine, 1-methylguanine, 3-methylcytidine, adenosine, methylthioadenosine sulfoxide, cytidine, and deoxycytidine. 1-Methyladenosine and 1-methylinosine were most consistently elevated in the urine of immunodeficient children. Methylthioadenosine sulfoxide was very markedly increased in urine of two of the immunodeficient children while more moderate increases were noted with a number of other nucleosides. The germ-free child with severe combined immunodeficiency showed consistently lower excretion levels of these compounds when compared to normal children.

Original languageEnglish (US)
Pages (from-to)37-51
Number of pages15
JournalBiochemical medicine
Volume34
Issue number1
DOIs
StatePublished - Aug 1985

ASJC Scopus subject areas

  • Biochemistry

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