Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group

Runglawan Silakit, Watcharin Loilome, Puangrat Yongvanit, Suyanee Thongchot, Nisana Namwat, Paiboon Sithithaworn, Thidarut Boonmars, Supinda Koonmee, Attapol Titapun, Narong Khuntikeo, Nittaya Chamadol, Anchalee Techasen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (PDF), which possibly associates to cholangiocarcinoma (CCA). Patients with CCA have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. Hence, detecting early stage CCA is essential. Secretory miRNAs have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. We aimed to determine levels of miR-192 and miR-21 in the urine of O. viverrini infected, periductal fibrosis (PDF) and CCA groups using qRT-PCR. We found that miR-192 was significantly higher in O. viverrini infected, PDF and also CCA groups (p < 0.05) than in healthy controls. By utilizing the Receiver Operation Characteristics (ROC) analysis, miR-192 differentiated patients with opisthorchiasis (the area under the curve; AUC = 0.766), PDF subjects (AUC = 0.781) and CCA patients (AUC = 0.682) from healthy controls. MiR-21 was significantly higher in PDF and CCA groups (p < 0.05) than in healthy controls. MiR-21 discriminated PDF subjects (AUC = 0.735) and CCA patients (AUC = 0.682) from healthy controls. Combined levels of these two miRNAs revealed an increased AUC of 0.812 for separating opisthorchiasis, AUC of 0.815 in discriminating PDF subjects, and AUC of 0.849 in differentiating CCA from healthy controls. Odds ratios (OR) indicated high levels of miR-192/miR-21 as risk predictors for opisthorchiasis, PDF and CCA. Levels of these miRNAs declined significantly for patients following praziquantel treatment. In conclusion, urinary miR-192/miR-21 have potential as risk indicators for opisthorchiasis and PDF-associated CCA in the endemic region.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalParasitology International
Volume66
Issue number4
DOIs
StatePublished - Aug 2017
Externally publishedYes

Fingerprint

Fasciola hepatica
Cholangiocarcinoma
MicroRNAs
Area Under Curve
Fibrosis
Opisthorchiasis
Opisthorchis
Asymptomatic Diseases
Praziquantel
Parasitic Diseases
Delayed Diagnosis
Bile Ducts
Biomarkers
Odds Ratio
Urine

Keywords

  • Cholangiocarcinoma
  • Opisthorchis viverrini
  • Periductal fibrosis
  • Risk indicator
  • Urinary microRNAs

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. / Silakit, Runglawan; Loilome, Watcharin; Yongvanit, Puangrat; Thongchot, Suyanee; Namwat, Nisana; Sithithaworn, Paiboon; Boonmars, Thidarut; Koonmee, Supinda; Titapun, Attapol; Khuntikeo, Narong; Chamadol, Nittaya; Techasen, Anchalee.

In: Parasitology International, Vol. 66, No. 4, 08.2017, p. 479-485.

Research output: Contribution to journalArticle

Silakit, R, Loilome, W, Yongvanit, P, Thongchot, S, Namwat, N, Sithithaworn, P, Boonmars, T, Koonmee, S, Titapun, A, Khuntikeo, N, Chamadol, N & Techasen, A 2017, 'Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group', Parasitology International, vol. 66, no. 4, pp. 479-485. https://doi.org/10.1016/j.parint.2015.10.001
Silakit, Runglawan ; Loilome, Watcharin ; Yongvanit, Puangrat ; Thongchot, Suyanee ; Namwat, Nisana ; Sithithaworn, Paiboon ; Boonmars, Thidarut ; Koonmee, Supinda ; Titapun, Attapol ; Khuntikeo, Narong ; Chamadol, Nittaya ; Techasen, Anchalee. / Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. In: Parasitology International. 2017 ; Vol. 66, No. 4. pp. 479-485.
@article{435ff1bf22b14957af04d864d019e35b,
title = "Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group",
abstract = "Opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (PDF), which possibly associates to cholangiocarcinoma (CCA). Patients with CCA have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. Hence, detecting early stage CCA is essential. Secretory miRNAs have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. We aimed to determine levels of miR-192 and miR-21 in the urine of O. viverrini infected, periductal fibrosis (PDF) and CCA groups using qRT-PCR. We found that miR-192 was significantly higher in O. viverrini infected, PDF and also CCA groups (p < 0.05) than in healthy controls. By utilizing the Receiver Operation Characteristics (ROC) analysis, miR-192 differentiated patients with opisthorchiasis (the area under the curve; AUC = 0.766), PDF subjects (AUC = 0.781) and CCA patients (AUC = 0.682) from healthy controls. MiR-21 was significantly higher in PDF and CCA groups (p < 0.05) than in healthy controls. MiR-21 discriminated PDF subjects (AUC = 0.735) and CCA patients (AUC = 0.682) from healthy controls. Combined levels of these two miRNAs revealed an increased AUC of 0.812 for separating opisthorchiasis, AUC of 0.815 in discriminating PDF subjects, and AUC of 0.849 in differentiating CCA from healthy controls. Odds ratios (OR) indicated high levels of miR-192/miR-21 as risk predictors for opisthorchiasis, PDF and CCA. Levels of these miRNAs declined significantly for patients following praziquantel treatment. In conclusion, urinary miR-192/miR-21 have potential as risk indicators for opisthorchiasis and PDF-associated CCA in the endemic region.",
keywords = "Cholangiocarcinoma, Opisthorchis viverrini, Periductal fibrosis, Risk indicator, Urinary microRNAs",
author = "Runglawan Silakit and Watcharin Loilome and Puangrat Yongvanit and Suyanee Thongchot and Nisana Namwat and Paiboon Sithithaworn and Thidarut Boonmars and Supinda Koonmee and Attapol Titapun and Narong Khuntikeo and Nittaya Chamadol and Anchalee Techasen",
year = "2017",
month = "8",
doi = "10.1016/j.parint.2015.10.001",
language = "English (US)",
volume = "66",
pages = "479--485",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",
number = "4",

}

TY - JOUR

T1 - Urinary microRNA-192 and microRNA-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group

AU - Silakit, Runglawan

AU - Loilome, Watcharin

AU - Yongvanit, Puangrat

AU - Thongchot, Suyanee

AU - Namwat, Nisana

AU - Sithithaworn, Paiboon

AU - Boonmars, Thidarut

AU - Koonmee, Supinda

AU - Titapun, Attapol

AU - Khuntikeo, Narong

AU - Chamadol, Nittaya

AU - Techasen, Anchalee

PY - 2017/8

Y1 - 2017/8

N2 - Opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (PDF), which possibly associates to cholangiocarcinoma (CCA). Patients with CCA have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. Hence, detecting early stage CCA is essential. Secretory miRNAs have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. We aimed to determine levels of miR-192 and miR-21 in the urine of O. viverrini infected, periductal fibrosis (PDF) and CCA groups using qRT-PCR. We found that miR-192 was significantly higher in O. viverrini infected, PDF and also CCA groups (p < 0.05) than in healthy controls. By utilizing the Receiver Operation Characteristics (ROC) analysis, miR-192 differentiated patients with opisthorchiasis (the area under the curve; AUC = 0.766), PDF subjects (AUC = 0.781) and CCA patients (AUC = 0.682) from healthy controls. MiR-21 was significantly higher in PDF and CCA groups (p < 0.05) than in healthy controls. MiR-21 discriminated PDF subjects (AUC = 0.735) and CCA patients (AUC = 0.682) from healthy controls. Combined levels of these two miRNAs revealed an increased AUC of 0.812 for separating opisthorchiasis, AUC of 0.815 in discriminating PDF subjects, and AUC of 0.849 in differentiating CCA from healthy controls. Odds ratios (OR) indicated high levels of miR-192/miR-21 as risk predictors for opisthorchiasis, PDF and CCA. Levels of these miRNAs declined significantly for patients following praziquantel treatment. In conclusion, urinary miR-192/miR-21 have potential as risk indicators for opisthorchiasis and PDF-associated CCA in the endemic region.

AB - Opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (PDF), which possibly associates to cholangiocarcinoma (CCA). Patients with CCA have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. Hence, detecting early stage CCA is essential. Secretory miRNAs have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. We aimed to determine levels of miR-192 and miR-21 in the urine of O. viverrini infected, periductal fibrosis (PDF) and CCA groups using qRT-PCR. We found that miR-192 was significantly higher in O. viverrini infected, PDF and also CCA groups (p < 0.05) than in healthy controls. By utilizing the Receiver Operation Characteristics (ROC) analysis, miR-192 differentiated patients with opisthorchiasis (the area under the curve; AUC = 0.766), PDF subjects (AUC = 0.781) and CCA patients (AUC = 0.682) from healthy controls. MiR-21 was significantly higher in PDF and CCA groups (p < 0.05) than in healthy controls. MiR-21 discriminated PDF subjects (AUC = 0.735) and CCA patients (AUC = 0.682) from healthy controls. Combined levels of these two miRNAs revealed an increased AUC of 0.812 for separating opisthorchiasis, AUC of 0.815 in discriminating PDF subjects, and AUC of 0.849 in differentiating CCA from healthy controls. Odds ratios (OR) indicated high levels of miR-192/miR-21 as risk predictors for opisthorchiasis, PDF and CCA. Levels of these miRNAs declined significantly for patients following praziquantel treatment. In conclusion, urinary miR-192/miR-21 have potential as risk indicators for opisthorchiasis and PDF-associated CCA in the endemic region.

KW - Cholangiocarcinoma

KW - Opisthorchis viverrini

KW - Periductal fibrosis

KW - Risk indicator

KW - Urinary microRNAs

UR - http://www.scopus.com/inward/record.url?scp=85020879618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020879618&partnerID=8YFLogxK

U2 - 10.1016/j.parint.2015.10.001

DO - 10.1016/j.parint.2015.10.001

M3 - Article

C2 - 26456596

AN - SCOPUS:85020879618

VL - 66

SP - 479

EP - 485

JO - Parasitology International

JF - Parasitology International

SN - 1383-5769

IS - 4

ER -