Primenenie metoda krioul'tramikrotomii s tsel'iu izucheniia ul'trastruktury khlamidiǐ.

Translated title of the contribution: Use of a cryoultramicrotomy method for the purpose of studying chlamydial ultrastructure

V. L. Popov, A. Viron, F. Eb, J. F. Léfebvre, J. Orfila

Research output: Contribution to journalArticle

Abstract

The method of cryoultramicrotomy was adapted for the study of the ultrastructure of HeLa and McCoy cells in monolayer cultures infected with Chlamydia, obligatory intracellular procaryotic parasites, the causative agents of ornithosis (strain Loth) and paratrachoma (strain LB 1). The cryosections were obtained by the fixation of the monolayer with 2.5% glutaraldehyde, by the gradual infiltration of precipitated cells with sucrose (0.6--1.2--1.8--2.3 M) prior to freezing in liquid nitrogen, and by the treatment of sections with 1% aqueous methyl cellulose solution before drying. This method ensured good preservation of both Chlamydia, in intracytoplasmic inclusions and host cells, as well as regular reproducibility of the results. Ultrathin sections showed a considerable polymorphism in the vegetative forms of Chlamydia, which was probably due to the structure of their cell walls. Chlamydia, were found to form small vesicle-like structures in the cavities of inclusions. The cell walls and granules inside the elementary bodies of the causative agent of ornithosis were stained with the use of phosphotungstic acid--HCl, pH 0.5.

Original languageUndefined
Pages (from-to)75-79
Number of pages5
JournalZhurnal Mikrobiologii Epidemiologii i Immunobiologii
Issue number3
StatePublished - Mar 1979
Externally publishedYes

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Cryoultramicrotomy
Chlamydia
Psittacosis
Cell Wall
Phosphotungstic Acid
Methylcellulose
Glutaral
HeLa Cells
Reproducibility of Results
Freezing
Sucrose
Parasites
Nitrogen

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Public Health, Environmental and Occupational Health

Cite this

Primenenie metoda krioul'tramikrotomii s tsel'iu izucheniia ul'trastruktury khlamidiǐ. / Popov, V. L.; Viron, A.; Eb, F.; Léfebvre, J. F.; Orfila, J.

In: Zhurnal Mikrobiologii Epidemiologii i Immunobiologii, No. 3, 03.1979, p. 75-79.

Research output: Contribution to journalArticle

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