Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs

Shradha Wali, Rishein Gupta, Ronald L. Veselenak, Yansong Li, Jieh Juen Yu, Ashlesh K. Murthy, Andrew P. Cap, M. Neal Guentzel, James P. Chambers, Guangming Zhong, Roger G. Rank, Richard Pyles, Bernard P. Arulanandam

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Guinea pigs have been used as a second animal model to validate putative antichlamydial vaccine candidates tested in mice. However, the lack of guinea pig-specific reagents has limited the utility of this animal model in Chlamydia sp. vaccine studies. Using a novel guinea pig-specific transcriptome array, we determined correlates of protection in guinea pigs vaccinated with Chlamydia caviae (C. caviae) via the intranasal route, previously reported by us and others to provide robust antigen specific immunity against subsequent intravaginal challenge. C. caviae vaccinated guinea pigs resolved genital infection by day 3 post challenge. In contrast, mock vaccinated animals continued to shed viable Chlamydia up to day 18 post challenge. Importantly, at day 80 post challenge, vaccinated guinea pigs experienced significantly reduced genital pathology - a sequelae of genital chlamydial infections, in comparison to mock vaccinated guinea pigs. Sera from vaccinated guinea pigs displayed antigen specific IgG responses and increased IgG1 and IgG2 titers capable of neutralizing GPIC in vitro. Th1-cellular/inflammatory immune genes and Th2-humoral associated genes were also found to be elevated in vaccinated guinea pigs at day 3 post-challenge and correlated with early clearance of the bacterium. Overall, this study provides the first evidence of guinea pig-specific genes involved in anti-chlamydial vaccination and illustrates the enhancement of the utility of this animal model in chlamydial pathogenesis.

Original languageEnglish (US)
Article numbere114261
JournalPLoS One
Volume9
Issue number12
DOIs
StatePublished - Dec 11 2014

Fingerprint

Transcriptome
transcriptome
guinea pigs
genitalia
Immunity
Guinea Pigs
Vaccination
Animals
immunity
vaccination
Immunoglobulin G
Genes
Infection
Chlamydia
infection
Vaccines
Antigens
Pathology
Animal Models
animal models

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs. / Wali, Shradha; Gupta, Rishein; Veselenak, Ronald L.; Li, Yansong; Yu, Jieh Juen; Murthy, Ashlesh K.; Cap, Andrew P.; Neal Guentzel, M.; Chambers, James P.; Zhong, Guangming; Rank, Roger G.; Pyles, Richard; Arulanandam, Bernard P.

In: PLoS One, Vol. 9, No. 12, e114261, 11.12.2014.

Research output: Contribution to journalArticle

Wali, S, Gupta, R, Veselenak, RL, Li, Y, Yu, JJ, Murthy, AK, Cap, AP, Neal Guentzel, M, Chambers, JP, Zhong, G, Rank, RG, Pyles, R & Arulanandam, BP 2014, 'Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs', PLoS One, vol. 9, no. 12, e114261. https://doi.org/10.1371/journal.pone.0114261
Wali, Shradha ; Gupta, Rishein ; Veselenak, Ronald L. ; Li, Yansong ; Yu, Jieh Juen ; Murthy, Ashlesh K. ; Cap, Andrew P. ; Neal Guentzel, M. ; Chambers, James P. ; Zhong, Guangming ; Rank, Roger G. ; Pyles, Richard ; Arulanandam, Bernard P. / Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs. In: PLoS One. 2014 ; Vol. 9, No. 12.
@article{467b966003a540e39ed0efa8b570e315,
title = "Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs",
abstract = "Guinea pigs have been used as a second animal model to validate putative antichlamydial vaccine candidates tested in mice. However, the lack of guinea pig-specific reagents has limited the utility of this animal model in Chlamydia sp. vaccine studies. Using a novel guinea pig-specific transcriptome array, we determined correlates of protection in guinea pigs vaccinated with Chlamydia caviae (C. caviae) via the intranasal route, previously reported by us and others to provide robust antigen specific immunity against subsequent intravaginal challenge. C. caviae vaccinated guinea pigs resolved genital infection by day 3 post challenge. In contrast, mock vaccinated animals continued to shed viable Chlamydia up to day 18 post challenge. Importantly, at day 80 post challenge, vaccinated guinea pigs experienced significantly reduced genital pathology - a sequelae of genital chlamydial infections, in comparison to mock vaccinated guinea pigs. Sera from vaccinated guinea pigs displayed antigen specific IgG responses and increased IgG1 and IgG2 titers capable of neutralizing GPIC in vitro. Th1-cellular/inflammatory immune genes and Th2-humoral associated genes were also found to be elevated in vaccinated guinea pigs at day 3 post-challenge and correlated with early clearance of the bacterium. Overall, this study provides the first evidence of guinea pig-specific genes involved in anti-chlamydial vaccination and illustrates the enhancement of the utility of this animal model in chlamydial pathogenesis.",
author = "Shradha Wali and Rishein Gupta and Veselenak, {Ronald L.} and Yansong Li and Yu, {Jieh Juen} and Murthy, {Ashlesh K.} and Cap, {Andrew P.} and {Neal Guentzel}, M. and Chambers, {James P.} and Guangming Zhong and Rank, {Roger G.} and Richard Pyles and Arulanandam, {Bernard P.}",
year = "2014",
month = "12",
day = "11",
doi = "10.1371/journal.pone.0114261",
language = "English (US)",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Use of a guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in guinea pigs

AU - Wali, Shradha

AU - Gupta, Rishein

AU - Veselenak, Ronald L.

AU - Li, Yansong

AU - Yu, Jieh Juen

AU - Murthy, Ashlesh K.

AU - Cap, Andrew P.

AU - Neal Guentzel, M.

AU - Chambers, James P.

AU - Zhong, Guangming

AU - Rank, Roger G.

AU - Pyles, Richard

AU - Arulanandam, Bernard P.

PY - 2014/12/11

Y1 - 2014/12/11

N2 - Guinea pigs have been used as a second animal model to validate putative antichlamydial vaccine candidates tested in mice. However, the lack of guinea pig-specific reagents has limited the utility of this animal model in Chlamydia sp. vaccine studies. Using a novel guinea pig-specific transcriptome array, we determined correlates of protection in guinea pigs vaccinated with Chlamydia caviae (C. caviae) via the intranasal route, previously reported by us and others to provide robust antigen specific immunity against subsequent intravaginal challenge. C. caviae vaccinated guinea pigs resolved genital infection by day 3 post challenge. In contrast, mock vaccinated animals continued to shed viable Chlamydia up to day 18 post challenge. Importantly, at day 80 post challenge, vaccinated guinea pigs experienced significantly reduced genital pathology - a sequelae of genital chlamydial infections, in comparison to mock vaccinated guinea pigs. Sera from vaccinated guinea pigs displayed antigen specific IgG responses and increased IgG1 and IgG2 titers capable of neutralizing GPIC in vitro. Th1-cellular/inflammatory immune genes and Th2-humoral associated genes were also found to be elevated in vaccinated guinea pigs at day 3 post-challenge and correlated with early clearance of the bacterium. Overall, this study provides the first evidence of guinea pig-specific genes involved in anti-chlamydial vaccination and illustrates the enhancement of the utility of this animal model in chlamydial pathogenesis.

AB - Guinea pigs have been used as a second animal model to validate putative antichlamydial vaccine candidates tested in mice. However, the lack of guinea pig-specific reagents has limited the utility of this animal model in Chlamydia sp. vaccine studies. Using a novel guinea pig-specific transcriptome array, we determined correlates of protection in guinea pigs vaccinated with Chlamydia caviae (C. caviae) via the intranasal route, previously reported by us and others to provide robust antigen specific immunity against subsequent intravaginal challenge. C. caviae vaccinated guinea pigs resolved genital infection by day 3 post challenge. In contrast, mock vaccinated animals continued to shed viable Chlamydia up to day 18 post challenge. Importantly, at day 80 post challenge, vaccinated guinea pigs experienced significantly reduced genital pathology - a sequelae of genital chlamydial infections, in comparison to mock vaccinated guinea pigs. Sera from vaccinated guinea pigs displayed antigen specific IgG responses and increased IgG1 and IgG2 titers capable of neutralizing GPIC in vitro. Th1-cellular/inflammatory immune genes and Th2-humoral associated genes were also found to be elevated in vaccinated guinea pigs at day 3 post-challenge and correlated with early clearance of the bacterium. Overall, this study provides the first evidence of guinea pig-specific genes involved in anti-chlamydial vaccination and illustrates the enhancement of the utility of this animal model in chlamydial pathogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84917690890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84917690890&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0114261

DO - 10.1371/journal.pone.0114261

M3 - Article

C2 - 25502875

AN - SCOPUS:84917690890

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e114261

ER -