Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

David W C Beasley, Michael R. Holbrook, Amelia P A Travassos Da Rosa, Lark Coffey, Anne Sophie Carrara, Kathrine Phillippi-Falkenstein, Rudolf P. Bohm, Marion S. Ratterree, Kristy M. Lillibridge, George V. Ludwig, Jose Estrada-Franco, Scott C. Weaver, Robert B. Tesh, Robert E. Shope, Alan D T Barrett

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

Original languageEnglish (US)
Pages (from-to)2759-2765
Number of pages7
JournalJournal of Clinical Microbiology
Volume42
Issue number6
DOIs
StatePublished - Jun 2004

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West Nile virus
Protein Subunits
Virus Diseases
Recombinant Proteins
Flavivirus
Antigens
St. Louis Encephalitis Viruses
Viral Envelope Proteins
Antibody Formation
Immune Sera
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. / Beasley, David W C; Holbrook, Michael R.; Travassos Da Rosa, Amelia P A; Coffey, Lark; Carrara, Anne Sophie; Phillippi-Falkenstein, Kathrine; Bohm, Rudolf P.; Ratterree, Marion S.; Lillibridge, Kristy M.; Ludwig, George V.; Estrada-Franco, Jose; Weaver, Scott C.; Tesh, Robert B.; Shope, Robert E.; Barrett, Alan D T.

In: Journal of Clinical Microbiology, Vol. 42, No. 6, 06.2004, p. 2759-2765.

Research output: Contribution to journalArticle

Beasley, DWC, Holbrook, MR, Travassos Da Rosa, APA, Coffey, L, Carrara, AS, Phillippi-Falkenstein, K, Bohm, RP, Ratterree, MS, Lillibridge, KM, Ludwig, GV, Estrada-Franco, J, Weaver, SC, Tesh, RB, Shope, RE & Barrett, ADT 2004, 'Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection', Journal of Clinical Microbiology, vol. 42, no. 6, pp. 2759-2765. https://doi.org/10.1128/JCM.42.6.2759-2765.2004
Beasley, David W C ; Holbrook, Michael R. ; Travassos Da Rosa, Amelia P A ; Coffey, Lark ; Carrara, Anne Sophie ; Phillippi-Falkenstein, Kathrine ; Bohm, Rudolf P. ; Ratterree, Marion S. ; Lillibridge, Kristy M. ; Ludwig, George V. ; Estrada-Franco, Jose ; Weaver, Scott C. ; Tesh, Robert B. ; Shope, Robert E. ; Barrett, Alan D T. / Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 6. pp. 2759-2765.
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