Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

David W C Beasley; Michael R. Holbrook; Amelia P A Travassos Da Rosa; Lark Coffey; Anne Sophie Carrara; Kathrine Phillippi-Falkenstein; Rudolf P. Bohm; Marion S. Ratterree; Kristy M. Lillibridge; George V. Ludwig; Jose Estrada-Franco; Scott C. Weaver; Robert B. Tesh; Robert E. Shope; Alan D T Barrett

doi:10.1128/JCM.42.6.2759-2765.2004

Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

David W C Beasley
, Michael R. Holbrook
, Amelia P A Travassos Da Rosa
, Lark Coffey
, Anne Sophie Carrara
, Kathrine Phillippi-Falkenstein
, Rudolf P. Bohm
, Marion S. Ratterree
, Kristy M. Lillibridge
, George V. Ludwig
, Jose Estrada-Franco
, Scott C. Weaver
, Robert B. Tesh
, Robert E. Shope
, Alan D T Barrett

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

Original language	English (US)
Pages (from-to)	2759-2765
Number of pages	7
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	6
DOIs	https://doi.org/10.1128/JCM.42.6.2759-2765.2004
State	Published - Jun 2004

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/JCM.42.6.2759-2765.2004

Cite this

Beasley, D. W. C., Holbrook, M. R., Travassos Da Rosa, A. P. A., Coffey, L., Carrara, A. S., Phillippi-Falkenstein, K., Bohm, R. P., Ratterree, M. S., Lillibridge, K. M., Ludwig, G. V., Estrada-Franco, J., Weaver, S. C., Tesh, R. B., Shope, R. E., & Barrett, A. D. T. (2004). Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. Journal of Clinical Microbiology, 42(6), 2759-2765. https://doi.org/10.1128/JCM.42.6.2759-2765.2004

Beasley, DWC, Holbrook, MR, Travassos Da Rosa, APA, Coffey, L, Carrara, AS, Phillippi-Falkenstein, K, Bohm, RP, Ratterree, MS, Lillibridge, KM, Ludwig, GV, Estrada-Franco, J, Weaver, SC, Tesh, RB, Shope, RE & Barrett, ADT 2004, 'Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection', Journal of Clinical Microbiology, vol. 42, no. 6, pp. 2759-2765. https://doi.org/10.1128/JCM.42.6.2759-2765.2004

@article{67186e3d74eb4ad28c05d61dad41f7de,

title = "Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection",

abstract = "Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.",

author = "Beasley, \{David W C\} and Holbrook, \{Michael R.\} and \{Travassos Da Rosa\}, \{Amelia P A\} and Lark Coffey and Carrara, \{Anne Sophie\} and Kathrine Phillippi-Falkenstein and Bohm, \{Rudolf P.\} and Ratterree, \{Marion S.\} and Lillibridge, \{Kristy M.\} and Ludwig, \{George V.\} and Jose Estrada-Franco and Weaver, \{Scott C.\} and Tesh, \{Robert B.\} and Shope, \{Robert E.\} and Barrett, \{Alan D T\}",

year = "2004",

month = jun,

doi = "10.1128/JCM.42.6.2759-2765.2004",

language = "English (US)",

volume = "42",

pages = "2759--2765",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

AU - Beasley, David W C

AU - Holbrook, Michael R.

AU - Travassos Da Rosa, Amelia P A

AU - Coffey, Lark

AU - Carrara, Anne Sophie

AU - Phillippi-Falkenstein, Kathrine

AU - Bohm, Rudolf P.

AU - Ratterree, Marion S.

AU - Lillibridge, Kristy M.

AU - Ludwig, George V.

AU - Estrada-Franco, Jose

AU - Weaver, Scott C.

AU - Tesh, Robert B.

AU - Shope, Robert E.

AU - Barrett, Alan D T

PY - 2004/6

Y1 - 2004/6

N2 - Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

AB - Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

UR - https://www.scopus.com/pages/publications/2942538171

UR - https://www.scopus.com/pages/publications/2942538171#tab=citedBy

U2 - 10.1128/JCM.42.6.2759-2765.2004

DO - 10.1128/JCM.42.6.2759-2765.2004

M3 - Article

C2 - 15184463

AN - SCOPUS:2942538171

SN - 0095-1137

VL - 42

SP - 2759

EP - 2765

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this