Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe county, New York

P. Yagupsky, M. Loeffelholz, K. Bell, M. A. Menegus

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Antibiotic susceptibility patterns, plasmid profiles, and endonuclease restriction analysis of plasmid DNA were used in the investigation of an epidemic of Shigella sonnei infections in Monroe County, New York, in 1988 and 1989. The epidemic peaked during the winter, included the simultaneous transmission of the disease from person to person and from common food sources, and especially affected inhabitants of the poor, inner-city neighborhoods, young children of both sexes, and women. Resistance to ampicillin, tetracycline, or trimethoprim-sulfamethoxazole, encoded in a 70-MDa plasmid, was found in most of the examined isolates. Unexpectedly, isolates from patients involved in a food-borne outbreak exhibited three different antibiotic susceptibility patterns, suggesting deletion of antibiotic resistance determinants in some strains. Antibiograms clearly separated food-borne outbreak-related and non-foodborne outbreak-related strains, distinguished more strains than did the plasmid profiles, and were useful in tracing the dissemination of individual isolates in the community. Restriction endonuclease analysis substantially increased the discriminatory value of plasmid profiles and validated the antibiogram results. The present study illustrates the complexity of epidemics of S. sonnei infections and shows the value of combining different biological markers in the investigation.

Original languageEnglish (US)
Pages (from-to)2850-2855
Number of pages6
JournalJournal of Clinical Microbiology
Volume29
Issue number12
StatePublished - 1991
Externally publishedYes

Fingerprint

Shigella sonnei
Plasmids
Disease Outbreaks
Infection
DNA Restriction Enzymes
Microbial Sensitivity Tests
Food
Anti-Bacterial Agents
Ampicillin Resistance
Sulfamethoxazole Drug Combination Trimethoprim
Microbial Drug Resistance
Tetracycline
Biomarkers
DNA

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe county, New York. / Yagupsky, P.; Loeffelholz, M.; Bell, K.; Menegus, M. A.

In: Journal of Clinical Microbiology, Vol. 29, No. 12, 1991, p. 2850-2855.

Research output: Contribution to journalArticle

Yagupsky, P. ; Loeffelholz, M. ; Bell, K. ; Menegus, M. A. / Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe county, New York. In: Journal of Clinical Microbiology. 1991 ; Vol. 29, No. 12. pp. 2850-2855.
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