Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing

Ping Ren, Sundara Sridhar, Vishnu Chaturvedi

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal primers revealed the presence of Saccharomyces cerevisiae.

Original languageEnglish (US)
Pages (from-to)2840-2842
Number of pages3
JournalJournal of Clinical Microbiology
Volume42
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)

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