Use of RNA interference to prevent lethal murine West Nile virus infection

Fengwei Bai, Tian Wang, Utpal Pal, Fukai Bao, L. Hannah Gould, Erol Fikrig

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge with an intraperitoneal inoculum of West Nile virus reduced the viral load and afforded partial protection from lethal infection. These data show the efficacy of the prophylactic use of siRNAs against a viral infection in vivo and suggest new strategies to combat West Nile virus.

Original languageEnglish (US)
Pages (from-to)1148-1154
Number of pages7
JournalJournal of Infectious Diseases
Volume191
Issue number7
DOIs
StatePublished - Apr 1 2005
Externally publishedYes

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West Nile virus
Virus Diseases
RNA Interference
Small Interfering RNA
Encephalitis
Hydrodynamics
Virus Replication
Viral Load
Vertebrates
Injections
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Use of RNA interference to prevent lethal murine West Nile virus infection. / Bai, Fengwei; Wang, Tian; Pal, Utpal; Bao, Fukai; Gould, L. Hannah; Fikrig, Erol.

In: Journal of Infectious Diseases, Vol. 191, No. 7, 01.04.2005, p. 1148-1154.

Research output: Contribution to journalArticle

Bai, Fengwei ; Wang, Tian ; Pal, Utpal ; Bao, Fukai ; Gould, L. Hannah ; Fikrig, Erol. / Use of RNA interference to prevent lethal murine West Nile virus infection. In: Journal of Infectious Diseases. 2005 ; Vol. 191, No. 7. pp. 1148-1154.
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