Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal

Tao Geng, Patricia K. Seitz, Mary L. Thomas, Bo Xu, Kizhake V. Soman, Alexander Kurosky, Bruce A. Luxon, Kathryn A. Cunningham

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) is an analytical technology for proteomic analysis that combines chromatography and mass spectrometry. At present, this technology is most commonly being exploited for the simultaneous measurement of numerous proteins in serum, but has also been utilized in organ tissue, although rarely in the brain. We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip® Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- versus saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectral pattern with six dominant peaks in all test samples. We have detected two smaller peaks (m/z: 5179, 5030) that were significantly increased (p < 0.05) in cocaine-treated rats compared to saline-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and has the potential to generate new hypotheses.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalJournal of Neuroscience Methods
Volume158
Issue number1
DOIs
StatePublished - Nov 15 2006

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Keywords

  • Brain
  • Cocaine
  • Mass spectrometry
  • Proteomics
  • SELDI
  • SELDI-TOF

ASJC Scopus subject areas

  • Neuroscience(all)

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