Use of transgenic mice to map cis-acting elements in the intestinal fatty acid binding protein gene (Fabpi) that control its cell lineage-specific and regional patterns of expression along the duodenal-colonic and crypt-villus axes of the gut epithelium

Steven Cohn, T. C. Simon, K. A. Roth, E. H. Birkenmeier, J. I. Gordon

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

The mouse intestinal epithelium is able to establish and maintain complex lineage-specific, spatial, and temporal patterns of gene expression despite its rapid and continuous renewal. A multipotent stem cell located near the base of each intestinal crypt gives rise to progeny which undergo amplification and allocation to either enterocytic, Paneth cell, goblet cell, or enteroendocrine cell lineages. Differentiation of these four lineages occurs during their geographically ordered migration along the crypt-villus axis. Gut stem cells appear to have a 'positional address' which is manifested by differences in the differentiation programs of their lineal descendants along the duodenal-colonic (cephalocaudal) axis. We have used the intestinal fatty acid binding protein gene (Fabpi) as a model to identify cis-acting elements which regulate cell- and region-specific patterns of gene expression in the gut. Nucleotides -1178 to +28 of rat Fabpi direct a pattern of expression of a reporter (human growth hormone [hGH]) which mimics that of mouse Fabpi (a) steady-state levels of hGH mRNA are highest in the distal jejunum of adult transgenic mice and fall progressively toward both the duodenum and the mid-colon; and (b) hGH is confined to the enterocytic lineage and first appears as postmitotic, differentiating cells exit the crypt and migrate to the base of small intestinal villi or their colonic homologs, the surface epithelial cuffs. Nucleotides -103 to +28, which are highly conserved in rat, mouse and human Fabpi, are able to correctly initiate transgene expression in late fetal life, restrict hGH to the enterocytic lineage, and establish an appropriate cephalocaudal gradient of reporter expression. This cephalocaudal gradient is also influenced by cis- acting elements located between nucleotides -1178 and -278, and -277 and -185 that enhance and suppress (respectively) expression in the ileum and colon and by element(s) located upstream of nucleotide -277 that are needed to sustain high levels of hGH production after weaning. Nucleotides -277 to -185 contain part of a domain conserved between the three orthologous Fabpi genes (nucleotides -240 to -159), a 24-bp element (nucleotides -212 to -188) that binds nuclear factors present in colonic but not small intestinal epithelial cells, and a portion of a CCAAT/enhancer binding protein footprint (C/EBPα, nucleotides -188 to -167). Removal of nucleotides -277 to -185 (yielding I- FABP(-184 to +28)/hGH +3) results in inappropriate expression of hGH in proliferating and nonproliferating epithelial cells located in the mid and upper portions of duodenal, jejunal, ileal, and colonic crypts without affecting the 'shape' of the cephalocaudal gradient of transgene expression. Despite this precocious induction of hGH production, the hGH reporter remains restricted to the enterocytic lineage in both the small intestine and colon suggesting that these replicating crypt epithelial cells may already be allocated to this lineage at the point of induction of transgene expression. Alternatively, these replicating, hGH-positive cells may not be fully committed to the enterocytic lineage but nucleotides -184 to +28 contain cis- acting elements that bind factors which mediate lineage-specific expression. Together, these results indicate Fabpi will be a useful model system for describing the molecular mechanisms that maintain axial pattern formation in the perpetually renewed, lineal descendants of the multipotent crypt stem cell.

Original languageEnglish (US)
Pages (from-to)27-44
Number of pages18
JournalJournal of Cell Biology
Volume119
Issue number1
StatePublished - Jan 1 1992
Externally publishedYes

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Fatty Acid-Binding Proteins
Human Growth Hormone
Cell Lineage
Transgenic Mice
Epithelium
Nucleotides
Genes
Transgenes
Multipotent Stem Cells
Colon
Epithelial Cells
Protein Footprinting
Paneth Cells
Enteroendocrine Cells
CCAAT-Enhancer-Binding Proteins
Gene Expression
Goblet Cells
Jejunum
Intestinal Mucosa
Protein C

ASJC Scopus subject areas

  • Cell Biology

Cite this

@article{e1d04b2441be4e7594cb1f22bb742876,
title = "Use of transgenic mice to map cis-acting elements in the intestinal fatty acid binding protein gene (Fabpi) that control its cell lineage-specific and regional patterns of expression along the duodenal-colonic and crypt-villus axes of the gut epithelium",
abstract = "The mouse intestinal epithelium is able to establish and maintain complex lineage-specific, spatial, and temporal patterns of gene expression despite its rapid and continuous renewal. A multipotent stem cell located near the base of each intestinal crypt gives rise to progeny which undergo amplification and allocation to either enterocytic, Paneth cell, goblet cell, or enteroendocrine cell lineages. Differentiation of these four lineages occurs during their geographically ordered migration along the crypt-villus axis. Gut stem cells appear to have a 'positional address' which is manifested by differences in the differentiation programs of their lineal descendants along the duodenal-colonic (cephalocaudal) axis. We have used the intestinal fatty acid binding protein gene (Fabpi) as a model to identify cis-acting elements which regulate cell- and region-specific patterns of gene expression in the gut. Nucleotides -1178 to +28 of rat Fabpi direct a pattern of expression of a reporter (human growth hormone [hGH]) which mimics that of mouse Fabpi (a) steady-state levels of hGH mRNA are highest in the distal jejunum of adult transgenic mice and fall progressively toward both the duodenum and the mid-colon; and (b) hGH is confined to the enterocytic lineage and first appears as postmitotic, differentiating cells exit the crypt and migrate to the base of small intestinal villi or their colonic homologs, the surface epithelial cuffs. Nucleotides -103 to +28, which are highly conserved in rat, mouse and human Fabpi, are able to correctly initiate transgene expression in late fetal life, restrict hGH to the enterocytic lineage, and establish an appropriate cephalocaudal gradient of reporter expression. This cephalocaudal gradient is also influenced by cis- acting elements located between nucleotides -1178 and -278, and -277 and -185 that enhance and suppress (respectively) expression in the ileum and colon and by element(s) located upstream of nucleotide -277 that are needed to sustain high levels of hGH production after weaning. Nucleotides -277 to -185 contain part of a domain conserved between the three orthologous Fabpi genes (nucleotides -240 to -159), a 24-bp element (nucleotides -212 to -188) that binds nuclear factors present in colonic but not small intestinal epithelial cells, and a portion of a CCAAT/enhancer binding protein footprint (C/EBPα, nucleotides -188 to -167). Removal of nucleotides -277 to -185 (yielding I- FABP(-184 to +28)/hGH +3) results in inappropriate expression of hGH in proliferating and nonproliferating epithelial cells located in the mid and upper portions of duodenal, jejunal, ileal, and colonic crypts without affecting the 'shape' of the cephalocaudal gradient of transgene expression. Despite this precocious induction of hGH production, the hGH reporter remains restricted to the enterocytic lineage in both the small intestine and colon suggesting that these replicating crypt epithelial cells may already be allocated to this lineage at the point of induction of transgene expression. Alternatively, these replicating, hGH-positive cells may not be fully committed to the enterocytic lineage but nucleotides -184 to +28 contain cis- acting elements that bind factors which mediate lineage-specific expression. Together, these results indicate Fabpi will be a useful model system for describing the molecular mechanisms that maintain axial pattern formation in the perpetually renewed, lineal descendants of the multipotent crypt stem cell.",
author = "Steven Cohn and Simon, {T. C.} and Roth, {K. A.} and Birkenmeier, {E. H.} and Gordon, {J. I.}",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "119",
pages = "27--44",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Use of transgenic mice to map cis-acting elements in the intestinal fatty acid binding protein gene (Fabpi) that control its cell lineage-specific and regional patterns of expression along the duodenal-colonic and crypt-villus axes of the gut epithelium

AU - Cohn, Steven

AU - Simon, T. C.

AU - Roth, K. A.

AU - Birkenmeier, E. H.

AU - Gordon, J. I.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The mouse intestinal epithelium is able to establish and maintain complex lineage-specific, spatial, and temporal patterns of gene expression despite its rapid and continuous renewal. A multipotent stem cell located near the base of each intestinal crypt gives rise to progeny which undergo amplification and allocation to either enterocytic, Paneth cell, goblet cell, or enteroendocrine cell lineages. Differentiation of these four lineages occurs during their geographically ordered migration along the crypt-villus axis. Gut stem cells appear to have a 'positional address' which is manifested by differences in the differentiation programs of their lineal descendants along the duodenal-colonic (cephalocaudal) axis. We have used the intestinal fatty acid binding protein gene (Fabpi) as a model to identify cis-acting elements which regulate cell- and region-specific patterns of gene expression in the gut. Nucleotides -1178 to +28 of rat Fabpi direct a pattern of expression of a reporter (human growth hormone [hGH]) which mimics that of mouse Fabpi (a) steady-state levels of hGH mRNA are highest in the distal jejunum of adult transgenic mice and fall progressively toward both the duodenum and the mid-colon; and (b) hGH is confined to the enterocytic lineage and first appears as postmitotic, differentiating cells exit the crypt and migrate to the base of small intestinal villi or their colonic homologs, the surface epithelial cuffs. Nucleotides -103 to +28, which are highly conserved in rat, mouse and human Fabpi, are able to correctly initiate transgene expression in late fetal life, restrict hGH to the enterocytic lineage, and establish an appropriate cephalocaudal gradient of reporter expression. This cephalocaudal gradient is also influenced by cis- acting elements located between nucleotides -1178 and -278, and -277 and -185 that enhance and suppress (respectively) expression in the ileum and colon and by element(s) located upstream of nucleotide -277 that are needed to sustain high levels of hGH production after weaning. Nucleotides -277 to -185 contain part of a domain conserved between the three orthologous Fabpi genes (nucleotides -240 to -159), a 24-bp element (nucleotides -212 to -188) that binds nuclear factors present in colonic but not small intestinal epithelial cells, and a portion of a CCAAT/enhancer binding protein footprint (C/EBPα, nucleotides -188 to -167). Removal of nucleotides -277 to -185 (yielding I- FABP(-184 to +28)/hGH +3) results in inappropriate expression of hGH in proliferating and nonproliferating epithelial cells located in the mid and upper portions of duodenal, jejunal, ileal, and colonic crypts without affecting the 'shape' of the cephalocaudal gradient of transgene expression. Despite this precocious induction of hGH production, the hGH reporter remains restricted to the enterocytic lineage in both the small intestine and colon suggesting that these replicating crypt epithelial cells may already be allocated to this lineage at the point of induction of transgene expression. Alternatively, these replicating, hGH-positive cells may not be fully committed to the enterocytic lineage but nucleotides -184 to +28 contain cis- acting elements that bind factors which mediate lineage-specific expression. Together, these results indicate Fabpi will be a useful model system for describing the molecular mechanisms that maintain axial pattern formation in the perpetually renewed, lineal descendants of the multipotent crypt stem cell.

AB - The mouse intestinal epithelium is able to establish and maintain complex lineage-specific, spatial, and temporal patterns of gene expression despite its rapid and continuous renewal. A multipotent stem cell located near the base of each intestinal crypt gives rise to progeny which undergo amplification and allocation to either enterocytic, Paneth cell, goblet cell, or enteroendocrine cell lineages. Differentiation of these four lineages occurs during their geographically ordered migration along the crypt-villus axis. Gut stem cells appear to have a 'positional address' which is manifested by differences in the differentiation programs of their lineal descendants along the duodenal-colonic (cephalocaudal) axis. We have used the intestinal fatty acid binding protein gene (Fabpi) as a model to identify cis-acting elements which regulate cell- and region-specific patterns of gene expression in the gut. Nucleotides -1178 to +28 of rat Fabpi direct a pattern of expression of a reporter (human growth hormone [hGH]) which mimics that of mouse Fabpi (a) steady-state levels of hGH mRNA are highest in the distal jejunum of adult transgenic mice and fall progressively toward both the duodenum and the mid-colon; and (b) hGH is confined to the enterocytic lineage and first appears as postmitotic, differentiating cells exit the crypt and migrate to the base of small intestinal villi or their colonic homologs, the surface epithelial cuffs. Nucleotides -103 to +28, which are highly conserved in rat, mouse and human Fabpi, are able to correctly initiate transgene expression in late fetal life, restrict hGH to the enterocytic lineage, and establish an appropriate cephalocaudal gradient of reporter expression. This cephalocaudal gradient is also influenced by cis- acting elements located between nucleotides -1178 and -278, and -277 and -185 that enhance and suppress (respectively) expression in the ileum and colon and by element(s) located upstream of nucleotide -277 that are needed to sustain high levels of hGH production after weaning. Nucleotides -277 to -185 contain part of a domain conserved between the three orthologous Fabpi genes (nucleotides -240 to -159), a 24-bp element (nucleotides -212 to -188) that binds nuclear factors present in colonic but not small intestinal epithelial cells, and a portion of a CCAAT/enhancer binding protein footprint (C/EBPα, nucleotides -188 to -167). Removal of nucleotides -277 to -185 (yielding I- FABP(-184 to +28)/hGH +3) results in inappropriate expression of hGH in proliferating and nonproliferating epithelial cells located in the mid and upper portions of duodenal, jejunal, ileal, and colonic crypts without affecting the 'shape' of the cephalocaudal gradient of transgene expression. Despite this precocious induction of hGH production, the hGH reporter remains restricted to the enterocytic lineage in both the small intestine and colon suggesting that these replicating crypt epithelial cells may already be allocated to this lineage at the point of induction of transgene expression. Alternatively, these replicating, hGH-positive cells may not be fully committed to the enterocytic lineage but nucleotides -184 to +28 contain cis- acting elements that bind factors which mediate lineage-specific expression. Together, these results indicate Fabpi will be a useful model system for describing the molecular mechanisms that maintain axial pattern formation in the perpetually renewed, lineal descendants of the multipotent crypt stem cell.

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