Use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever

J. I. Herrero-Herrero, David Walker, R. Ruiz-Beltrán

Research output: Contribution to journalArticle

Abstract

The reactivity of antigenic components of Rickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100 % and 86.7 %, respectively, of acute sera and with 100 % of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7 % of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.

Original languageEnglish (US)
Pages (from-to)939-942
Number of pages4
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Volume11
Issue number10
DOIs
StatePublished - Oct 1992

Fingerprint

Boutonneuse Fever
Western Blotting
Antigens
Serum
Rickettsia conorii
Humoral Immunity
Lipopolysaccharides
Molecular Weight
Staining and Labeling

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

Cite this

Use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever. / Herrero-Herrero, J. I.; Walker, David; Ruiz-Beltrán, R.

In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 11, No. 10, 10.1992, p. 939-942.

Research output: Contribution to journalArticle

@article{72baf2f55d654facabc472790fc7a377,
title = "Use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever",
abstract = "The reactivity of antigenic components of Rickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100 {\%} and 86.7 {\%}, respectively, of acute sera and with 100 {\%} of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7 {\%} of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.",
author = "Herrero-Herrero, {J. I.} and David Walker and R. Ruiz-Beltr{\'a}n",
year = "1992",
month = "10",
doi = "10.1007/BF01962380",
language = "English (US)",
volume = "11",
pages = "939--942",
journal = "European Journal of Clinical Microbiology and Infectious Diseases",
issn = "0934-9723",
publisher = "Vieweg",
number = "10",

}

TY - JOUR

T1 - Use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever

AU - Herrero-Herrero, J. I.

AU - Walker, David

AU - Ruiz-Beltrán, R.

PY - 1992/10

Y1 - 1992/10

N2 - The reactivity of antigenic components of Rickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100 % and 86.7 %, respectively, of acute sera and with 100 % of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7 % of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.

AB - The reactivity of antigenic components of Rickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100 % and 86.7 %, respectively, of acute sera and with 100 % of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7 % of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.

UR - http://www.scopus.com/inward/record.url?scp=0027053806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027053806&partnerID=8YFLogxK

U2 - 10.1007/BF01962380

DO - 10.1007/BF01962380

M3 - Article

VL - 11

SP - 939

EP - 942

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

IS - 10

ER -