USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.

Annette Aichem, Christiane Pelzer, Sebastian Lukasiak, Birte Kalveram, Paul W. Sheppard, Neha Rani, Gunter Schmidtke, Marcus Groettrup

Research output: Contribution to journalArticle

Abstract

The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred from UBA6 onto USE1 in vitro, and endogenous USE1 and FAT10 can be coimmunoprecipitated from intact cells. Small interfering RNA-mediated downregulation of USE1 mRNA resulted in a strong reduction of FAT10 conjugate formation under endogenous conditions, suggesting that USE1 is a major E2 enzyme in the FAT10 conjugation cascade. Interestingly, USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans.

Original languageEnglish (US)
Pages (from-to)13
Number of pages1
JournalNature Communications
Volume1
StatePublished - 2010
Externally publishedYes

Fingerprint

Ubiquitin-Conjugating Enzymes
enzymes
Enzymes
conjugation
Proteasome Endopeptidase Complex
Ubiquitin
Small Interfering RNA
Proteolysis
cascades
Down-Regulation
degradation
proteins
Degradation
Messenger RNA
Substrates
cells
Proteins
interactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Aichem, A., Pelzer, C., Lukasiak, S., Kalveram, B., Sheppard, P. W., Rani, N., ... Groettrup, M. (2010). USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. Nature Communications, 1, 13.

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. / Aichem, Annette; Pelzer, Christiane; Lukasiak, Sebastian; Kalveram, Birte; Sheppard, Paul W.; Rani, Neha; Schmidtke, Gunter; Groettrup, Marcus.

In: Nature Communications, Vol. 1, 2010, p. 13.

Research output: Contribution to journalArticle

Aichem, A, Pelzer, C, Lukasiak, S, Kalveram, B, Sheppard, PW, Rani, N, Schmidtke, G & Groettrup, M 2010, 'USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.', Nature Communications, vol. 1, pp. 13.
Aichem A, Pelzer C, Lukasiak S, Kalveram B, Sheppard PW, Rani N et al. USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. Nature Communications. 2010;1:13.
Aichem, Annette ; Pelzer, Christiane ; Lukasiak, Sebastian ; Kalveram, Birte ; Sheppard, Paul W. ; Rani, Neha ; Schmidtke, Gunter ; Groettrup, Marcus. / USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis. In: Nature Communications. 2010 ; Vol. 1. pp. 13.
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